10-羟基喜树碱对异基因大鼠心脏移植物急性排斥反应的抑制作用  

Inhibition of acute rejection of allogenic heart transplantation in rats by HCPT

作　　者：莫春柏[1] 石炳毅[1] 蔡明[1] 钱叶勇[1] 周文强[1] 王燕[1] 

机构地区：[1]解放军三零九医院器官移植中心,北京100091

出　　处：《中华器官移植杂志》2002年第6期347-349,共3页Chinese Journal of Organ Transplantation

摘　　要：目的　探讨 10 羟基喜树碱 (HCPT)对移植物急性排斥反应的免疫抑制作用。方法　以SD大鼠为供者 ,Wistar大鼠为受者 ,行大鼠同种异体异位心脏移植。用中药制剂 10 羟基喜树碱(HCPT)预防和治疗移植物急性排斥反应。结果　HCPT 1.0mg·kg-1·d-1组与对照组比较 ,移植物存活时间明显延长 (6.0 5d/ 11.45d ,P <0 .0 0 1) ;HCPT 2 .0mg·kg-1·d-1组作用更为显著 (>2 41.6d) ,其中 3只大鼠于术后 60d停用HCPT ,移植心脏长期存活 ,超过 73 0d ,并经证实已形成免疫耐受。大鼠心脏移植物发生急性排斥反应时 ,HCPT 10 .0mg·kg-1·d-1能使排斥反应完全逆转 ,移植心脏功能完全恢复正常。较大剂量HCPT可导致轻微的胃肠道反应 ,但未观察到明显的肝、肾、心、脾、睾丸和造血系统损害。结论　HCPT对大鼠移植心脏急性排斥反应具有显著的抑制作用 。Objective To investigate the immunoinhibitory effects of HCPT in treating acute rejection of allogenic heart transplantation in rat. Methods The inbred SD rats were chosen as donors and inbred Wistar rats as recipients. Overall, 40 cervical heterotopic heart transplants were performed. The 40 recipients were classified into 4 groups randomly. After heart transplantation, the rats in the group A received placebo; group B, HCPT 1.0?mg/kg (i.p) every day; group C, HCPT 2.0?mg/kg (i.p) every day; group D, 10 rats received placebo in the initial 4?days after transplantation, afterwards, when the acute rejection happened, 10?mg/kg of HCPT every day was given (i.p) for 3?days. Results MST in the groups A, B and C was 6.05?days, 11.45?days and over 241.6?days. Three heart grafts in the group C survived more than 730?days even HCPT was withdrawn at day 60?after transplantation and tolerance was proved in these rats. In the group D, after acute rejection was treated with high dose of HCPT, ( 4.85± 0.85)days later,all the acute rejection was reversed, and the normal heart beating of the grafts recovered. Otherwise, high dose of HCPT could result in moderate gastrointestinal disorders, and no damage to liver, kidney, heart, spleen, testis and hematopoietic system was observed. Conclusion HCPT could prevent and reverse acute rejection of allogenic heart transplantation in rats effectively and HCPT therapy might provide a novel strategy to inhibit acute rejection of allograft.

关 键 词：10-羟基喜树碱 急性排斥反应 抑制作用 心脏移植 大鼠 

分 类 号：R654.2[医药卫生—外科学]