电针对胃粘膜损伤大鼠血管活性肠肽影响的实验研究  

EXPERIMENTAL STUDY ON THE EFFECT OF ELECTROACUPUNCTURE ON VASOACTIVE INTENSTINAL PEPTIDE IN RAT WITH GASTRIC MUCOSAL DAMAGE

作　　者：申国明[1] 江安宏[1] 徐颖[1] 吕磊[1] 何峰[2] 许冠荪[2] 

机构地区：[1]安徽中医学院中西医结合系,合肥230038 [2]安徽中医学院针灸经济研究所,合肥230038

出　　处：《中国中医药科技》2002年第6期321-322,共2页Chinese Journal of Traditional Medical Science and Technology

摘　　要：目的 :观察电针足三里对胃粘膜损伤大鼠血管活性肠肽的影响。方法 :采用冷冻 -束缚应激致大鼠胃粘膜损伤模型 ,氢气清除法检测胃粘膜血流量 ,放射免疫分析法测定电针前后外周血、胃粘膜和脑组织血管活性肠肽含量。结果 :冷冻 -束缚应激大鼠胃粘膜损伤明显 ,胃粘膜出现点状条状出血坏死灶 ,尤以胃窦部为主 ,GMBF明显下降 (P <0 0 1) ,血浆、胃粘膜和脑组织中VIP含量均显著下降 (P <0 0 5 ,与正常组比较 ) ;电针刺激足三里穴后 ,GMBF显著上升 (P <0 0 1) ,LI显著降低 (P <0 0 5 ) ,血、胃粘膜和脑组织VIP含量增加 (P <0 0 1)。结论 :电针足三里穴在对胃粘膜损伤发挥保护作用的同时 ,可引起血管活性肠肽含量变化 。Objective:To study the effect of electroacupuncture(EA) of Zusanli (ST 36) on vasoactive intestinal peptide(VIP)levels in rats with gastric mucosal damage.Methods:40 Wistar rats were randomly divided into 4 groups:normal control group,model group(group with gastric mucosal damage,GMD),EA group(with EA Zuasnli +GMD),non-point group(with EA+non-point+GMD).The model of gastric mucosal damage was induced by restrained-cold stress.Gsatric mucosal blood flow(GMBF)were measured by the method of 'hydrogen gas clear'.Contents of VIP in plasma,antrum and medulla were determined by radiooimmunoassay. Results:In comparison with control group,GMBF of model group decreased significantly(P<0 01);while contents of VIP in plasma,antrum and medulla lowered obviously(P<0 05).Compared with model group,contents of VIP and GMBF in EA group increased significantly(P<0 01);whereas LI decreased obviously(P<0 01) Conclusion:EA at Zusanli (st36) can protect gastric mucosa from injury in stress rats.VIP may play a certain role in the protective effect of electroacupuncture on the gastric mucosal damage.

关 键 词：胃粘膜损伤 大鼠 针灸疗法 血管活性肠肽 实验研究 

分 类 号：R245.97[医药卫生—针灸推拿学]