妊娠高血压综合征患者胎盘还原型烟酰胺腺嘌呤二核苷酸脱氢酶基因的表达  

Expression of nicotinamide edenine dinucleotide dehydrogenase gene in placenta of patients with pregnancy induced hypertension

作　　者：杨丽媛[1] 姚元庆[1] 时永全[2] 王新[2] 师娟子[1] 

机构地区：[1]第四军医大学唐都医院妇产科,西安710038 [2]第四军医大学西京医院消化疾病研究所,西安710038

出　　处：《中华妇产科杂志》2002年第11期660-662,共3页Chinese Journal of Obstetrics and Gynecology

基　　金：国家自然科学基金资助项目 (30 0 70 787)

摘　　要：目的　研究还原型烟酰胺腺嘌呤二核苷酸 (NADH)脱氢酶基因在妊娠高血压综合征 (妊高征 )患者胎盘组织中的表达 ,了解其在妊高征发病中的作用。方法　采用32 P标记的NADH脱氢酶cDNA探针斑点印迹杂交方法 ,对 10例正常妊娠妇女 (对照组 )及 10例中、重度妊高征患者 (妊高征组 )的胎盘组织总RNA进行检测 ,以图像处理系统测量每个杂交点的平均吸光度 (A)值 ,比较两组胎盘组织NADH脱氢酶mRNA水平。结果　妊高征组和对照组胎盘组织NADH脱氢酶平均A值分别为 2 1± 6、5 6± 16;妊高征组胎盘组织NADH脱氢酶mRNA的表达明显高于对照组 ,两组比较 ,差异有显著性 (P <0 0 5 )。结论　妊高征患者胎盘组织NADH脱氢酶基因表达的增加 。Objective To investigate the expression of nicotinamide edenine dinucleotide (NADH) dehydrogenase gene in the placenta of patients with pregnancy induced hypertension (PIH) and the role of NADH dehyodrogenase in PIH pathogenesis Methods Using 32 P dATP labeled human NADH dehydrogenase cDNA probe, the expression of NADH dehydrogenase gene in placental tissues of 10 normal late pregnant (NLP) women and 10 PIH patients was detected by dot blot Mean A of each hybridization dot was measured by image analysis system to compare NADH dehydrogenase mRNA level in PIH placentae with that in NLP placenta Results The NADH dehydrogenase gene values in patients with PIH and NLP were 21±6 and 56±16 The level of NADH dehydrogenase mRNA in PIH placenta was significantly higher than that in normal palcentae ( P <0 05) Conclusion Elevated expression of NADH dehydrogenase gene may play a role in PIH pathogenesis

关 键 词：妊娠高血压综合征 心血管 胎盘 NADH脱氢酶 

分 类 号：R714.246[医药卫生—妇产科学]