中药补肾益气活血作用对生长受限胎鼠脑及肝细胞外信号调节激酶-1与丝裂素活化蛋白激酶磷酸酶-1表达的影响  

Effect of traditional Chinese medicine on expression of extracellular signal-regulating kinase-1 and mitogen activated protein kinase phosphatase-1 in brain and liver of fetal rats with fetal growth restriction

作　　者：吴云霞[1] 黄光英[1] 李靖 

机构地区：[1]华中科技大学同济医学院中西医结合研究所,武汉430030 [2]武装警察部队湖北省总队医院急诊科

出　　处：《中华妇产科杂志》2002年第11期672-675,共4页Chinese Journal of Obstetrics and Gynecology

基　　金：国家自然科学基金资助项目 (39870 92 3)

摘　　要：目的　探讨补肾益气活血方剂对生长受限胎鼠脑及肝细胞外信号调节激酶 1(ERK 1)和丝裂素活化蛋白激酶磷酸酶 1(MKP 1)表达的影响。方法　采用被动吸烟法建立孕鼠的生长受限模型 ,对 48只孕鼠随机分成生长受限组 (12只 )、精氨酸治疗组 (12只 )、补肾益气活血方剂 (简称中药 )中药治疗组 (12只 ) ,另选 12只正常孕鼠组成正常组。运用Western blot方法 ,分别检测各组孕鼠所生产胎鼠的脑组织及肝组织内ERK 1和MKP 1的表达。结果　 (1)脑组织 :生长受限组、精氨酸治疗组、中药治疗组和正常组ERK 1的表达分别为 7 63± 0 2 2、10 0 3± 0 41、11 0 3± 0 3 0、11 44± 0 0 9;MKP 1的表达分别为 7 41± 0 3 8、10 3 5± 0 60、10 60± 0 14、11 60± 0 62。 (2 )肝组织 :生长受限组、精氨酸治疗组、中药治疗组和正常组ERK 1的表达分别为 4 73± 0 5 4、5 83± 0 17、11 3 7± 0 12、12 3 4± 0 14;MKP 1的表达分别为 9 0 7± 0 61、10 66± 0 0 8、14 2 7± 0 73、14 92± 0 17。 (3 )在肝组织和脑组织中 ,生长受限组ERK 1和MKP 1的表达较正常组明显降低 (P <0 0 1) ,中药治疗组较生长受限组明显升高 (P <0 0 1) ,且接近于正常组的表达水平 (P >0 0 5 )。脑组织ERK 1的表达 ,中药治?Objective To investigate the effect of reinforcing kidney, replenishing Qi and blood activating prescription(RKRQBAP) on extracellular signal regulating kinase 1 (ERK 1) and mitogen activated protein kinase phosphatase 1 (MKP 1 ) of fetal rats with fetal growth restriction(FGR) Methods Passive smoking was used to establish animal model of FGR All pregnant rats were divided into four groups: FGR group, L arg group: rats with FGR treated with L argine, Chinese medicine group (CHM group): rats with FGR treated by traditional Chinese medicine and normal control group The expression of ERK 1 and MKP 1 in brain and liver were measured by western blot Results (1) According to FGR group, L arg group, CHM group and normal group, the expression of ERK 1 in brain was 7 63±0 22, 10 03±0 41, 11 03±0 30, 11 44±0 09 and MKP 1 was 7 41±0 38, 10 35±0 60, 10 60±0 14, 11 60±0 62 (2) The expression of ERK 1 in liver was 4 73±0 54, 5 83±0 17, 11 37±0 12, 12 34±0 14 accordingly and MKP 1 was 9 07±0 61, 10 66±0 08, 14 27±0 73, 14 92±0 17 (3) The expression of ERK 1 and MKP 1 in brain and liver was much lower in FGR group than in normal group ( P <0 01), and that of CHM group was much higher than in FGR group( P <0 01) and closed to normal group( P >0 05) The expression of ERK 1 in brain was much higher in CHM group than in L arg group ( P <0 05), the expression of MKP 1 was almost the same( P >0 05); Both of them in liver were higher in CHM group than in L arg group( P <0 01) Conclusion The possible mechanism which the RKRQBAP cure FGR is to affect the expression of ERK 1 and MKP 1 and to promote growth related genes expression and restrain apoptosis In addition, RKRQBAP is superior to L argine in treatment of FGR

关 键 词：胎儿生长迟缓 补肾 益气活血 有丝分裂素激活蛋白激酶类 蛋白质酪氨酸磷酸酶 即早蛋白质类 

分 类 号：R285.5[医药卫生—中药学]