芎归滴丸对海马神经元钠电流的保护作用  

Protective effect of Xionggui Diwan on sodium current in hippocampal neurons

作　　者：刘卫[1] 刘玉玲[2] 吕泰省[1] 刘传缋[3] 俞惠琴[1] 

机构地区：[1]解放军第88医院药剂科,山东泰安271000 [2]泰安市药品检验所,山东泰安271000 [3]军事医学科学院毒物药物研究所,北京100850

出　　处：《中国药学杂志》2002年第11期824-826,共3页Chinese Pharmaceutical Journal

基　　金：国家新药研究基金 (No 98-X -0 5 )

摘　　要：目的　观察新药芎归滴丸对海马神经元是否有保护作用。方法　用膜片箝全细胞记录方法检测缺氧 复氧前后体外培养的大鼠海马神经元的电压门控性Na+ ,K+ 电流。结果　急性缺氧 复氧后海马神经元的电压门控性Na+ 电流幅度显著降低 ;K+ 电流幅度无显著改变。芎归滴丸预处理的海马神经元经缺氧 复氧后Na+ ,K+ 电流幅度都没有显著降低。结论　在不损伤K+ 电流的缺氧条件下 ,芎归滴丸预处理能使海马神经元的电压门控性Na+OBJECTIVE: To investigate whether Xionggui Diwan protects hippocampal neurons from anoxia. METHODS: It was monitored voltage-dependent Na+ -K+ currents in cultured hippocampal in vitro before and after anoxia/recovery treatment by means of whole-cell recording patch clamp technique. RESULTS: Na+ currents was decreased significantly due to acute anoxia/treatment in hippocampal neurons, while K+ currents did not change singnificantly. Both K+ and Na+ currents in hippocampal neurons pretreated by Xionggui Diwan were not decreased via acute anoxia/treatment. CONCLUSION: Xionggui Diwan pretreatment can protect hippocampal neurons from moderate anoxia damage.

关 键 词：保护作用 海马神经元 缺氧 全细胞记录 芎归滴丸 中药 药理 

分 类 号：R285.5[医药卫生—中药学]