钙化心肌细胞肾上腺髓质素和受体活性修饰蛋白2mRNA含量的变化  

Changes of Amount of Adrneomedullin mRNA and Receptor Activity Modifying Protein 2 mRNA in Calcified Myocardial Cells

作　　者：齐永芬[1] 牛大地[2] 王述女亘 卜定方[1] 石彦荣[1] 高霖[2] 蒋宏峰[2] 庞永正[1] 唐朝枢[1] 

机构地区：[1]北京大学第一医院心血管所,北京市100034 [2]北京大学医学部生理系,北京市100083

出　　处：《中国动脉硬化杂志》2002年第5期375-378,共4页Chinese Journal of Arteriosclerosis

基　　金：国家重大基础研究发展规划项目 (G2 0 0 0 0 5 690 5 )资助

摘　　要：为探讨离体钙化处理的新生大鼠心肌细胞肾上腺髓质素及受体活性修饰蛋白 2mRNA含量的改变及意义。采用β 磷酸甘油制备钙化的心肌细胞 ;放射免疫分析方法测定心肌细胞中肾上腺含量 ;竞争性定量逆转录—多聚酶链反应测定心肌细胞肾上腺髓质素和受体活性修饰蛋白 2mRNA水平。结果显示 ,钙化心肌细胞钙含量较非钙化心肌细胞高 3倍 (P <0 .0 1) ,肾上腺髓质素含量较非钙化心肌细胞高 136 .9% (P <0 .0 1) ,钙化心肌细胞肾上腺髓质素和受体活性修饰蛋白 2mRNA含量分别增加 2 4 % (P <0 .0 5 )和 2 5 % (P <0 .0 5 )。而且钙化心肌细胞受体活性修饰蛋白 2和肾上腺髓质素基因表达上调的程度相平行。钙化心肌细胞肾上腺髓质素生成增加 ,肾上腺髓质素和受体活性修饰蛋白 2mRNA表达亦明显上调 ,提示肾上腺髓质素 受体活性修饰蛋白 2信号途径在心肌细胞钙化发展中可能具有一定的调节作用。Aim To explore the changes and significance of adrenomedullin(ADM) mRNA and receptor activity modifying protein 2(RAMP2) mRNA in calcified myocardial cells. Methods Calcified myocardial cells was prepared with β glycerophosphate. Content of ADM in VSMC was measured by radioimmunoassay(RIA). The amount of ADM mRNA and RMAP2 mRNA was determined by competitive RT PCR. Results The content of calcium and ADM in calcified myocardial cells was increased by 138% and 136.9%(all P<0.01), respectively, compared with control. The amount of ADM mRNA and RMAP2 mRNA in calcified myocardial cells was elevated by 24% and 25%( all P<0.05), respectively, compared with control. The elevated level of RAMP2 mRNA was parallel to that of ADM mRNA in calcified myocardial cells. Conclusions Content of ADM was increased, and gene expression of ADM and RAMP2 was up regulated in calcified myocardial cells, which suggested that ADM/RAMP2 may have important regulator role in process of calcification of myocardidal cells.

关 键 词：心肌细胞 钙化 肾上腺髓质素 受体活性修饰蛋白2 MRNA 

分 类 号：R542.2[医药卫生—心血管疾病]