免疫金银染色观察缺血再灌注损伤后肾小管整合素α3分布改变  

Changes of distribution of tubular α3 integrins after renal ischemia/reperfusion injury

作　　者：刘宏[1] 吴雄飞[1] 金锡御[1] 

机构地区：[1]第三军医大学西南医院泌尿中心肾科,重庆400038

出　　处：《重庆医学》2002年第11期1035-1036,共2页Chongqing medicine

基　　金：国家自然科学基金资助项目 (39870 348)

摘　　要：目的　观察在体缺血再灌注损伤对肾小管上皮细胞整合素α3分布的影响。方法　采用整合素α3抗血清 ,对比使用常规免疫组化和免疫金银染色。结果　免疫金银法染色显示 ,正常肾小管上皮细胞整合素α3仅于细胞基底侧极性分布 ,再灌注后细胞顶端出现整合素α3银染颗粒。结论　再灌注后肾小管整合素α3出现由基底侧向顶端的弥散 ,这一改变有可能对上皮细胞的脱落和小管梗阻产生影响。Objective To observe the effects of in vivo ischemia/reperfusion injury on the distribution of integrin α3 in tubular cells.Methods Goat anti rat integrin α3 antiserum were used as primary antibody, and the method of traditional immunohistochemistry and immunogold silver staining were compared.Results Using immunogold sliver staining method, it was revealed that the silver particles were distributed only in basal surfaces of tubular cells but appeared in apical surfaces after ischemia/reperfusion injury. Conclusions Reorganization of α3 integrins from basal to apical surfaces of tubular cells after reperfusion may participated in tubular exfoliation and tubular obstruction.

关 键 词：免疫金银法 缺血再灌注 整合素 

分 类 号：R692.6[医药卫生—泌尿科学]