制备皱纹盘鲍染色体标本方法的比较研究  

Chromosome ploidy test technique of Haliotis discus Hannai

作　　者：李雅娟[1] 毛连菊[1] 王子臣[1] 

机构地区：[1]大连水产学院农业部海洋水产增养殖生态学重点开放实验室,辽宁大连116023

出　　处：《大连水产学院学报》2002年第4期297-300,共4页Journal of Dalian Fisheries University

基　　金：国家海洋技术研究计划项目资助(海洋"863"计划(863-819-01-04)

摘　　要：以皱纹盘鲍HaliolisdiscusHannai的鳃、外套膜、上足触手为材料,采用植物血球凝集素(PHA)和秋水仙素体内注射法,通过滴片制备染色体标本,统计细胞分裂数。结果表明,鳃、外套膜、上足触手3种组织注射PHA后,细胞分裂指数比没有注射PHA的对照组均有显著提高,其中以PHA2次注射为最高。在3种组织中,鳃组织的细胞分裂指数最高,其次是外套膜、上足触手。用3种组织制备的染色体标本,均获得大量形态清晰、长度适中、分散良好的中期分裂相。The injection of PHA and Colchicine and chopping method were used for chromosome preparation from gill, mantle and epipodial tentacles of Pacific ablone Haliotis discus Hannai. The results indicated that the cell mitotic index increased after injection of PHA. The normal metaphase was observed in cells from three kinds of tissue. In this simple method,the mantle and opipodial tentacle were used in vitro for chromesome polyploid breeding of ablone.

关 键 词：比较研究 皱纹盘鲍 染色体标本 制备方法 植物益球凝集素 秋水仙素 

分 类 号：Q959.223.5[生物学—动物学]