重组人生长激素对梗阻性黄疸作用的实验研究  

Experimental Research of Recombinant Human Growth Hormone on Obstructive Jaundice

作　　者：王彤[1] 姜秀峰[2] 王琛[1] 寇志民[1] 

机构地区：[1]兰州医学院第二附属医院普外科,兰州730030 [2]甘肃省人民医院,兰州730000

出　　处：《中国普外基础与临床杂志》2002年第6期408-411,共4页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的　观察重组人生长激素 (rhGH)应用于梗阻性黄疸 (梗黄 )及其行内、外引流术后的治疗作用。方法　随机将新西兰白兔分为梗黄内引流 +rhGH治疗组、梗黄内引流 +生理盐水 (NS)组、梗黄外引流 +rhGH治疗组及梗黄外引流 +NS组。rhGH组在梗黄后开始皮下注射rhGH 0 .2IU kg ,每天两次 ,对照组则皮下注射相同容量的NS。分别在建立梗黄模型前以及梗黄模型后 14d及内、外引流后 14d测定动物的生化全套、内毒素、肿瘤坏死因子、可溶性白细胞介素 2受体以及营养状况的改变。结果　梗黄后施行内、外引流 4d后 ,治疗组与对照组相比体重增加 (P<0 .0 5 ) ,梗黄后 7d、10d ,治疗组与对照组相比血糖升高 (P<0 .0 5 )。梗黄后 14d ,治疗组与对照组相比血清白蛋白、转铁蛋白、前蛋白明显升高 (P<0 .0 1) ;血清总胆固醇、低密度脂蛋白、总胆汁酸 ,治疗组与对照组相比明显降低 (P<0 .0 5 ) ;血清谷草转氨酶、谷氨酰转移酶、总胆红素、尿素氮、肌酐、尿酸 ,治疗组与对照组相比明显降低 (P<0 .0 5 )。Ca2 + 治疗组与对照组相比明显升高 (P<0 .0 5 ) ;外引流后 14d ,K+ 、Na+ 治疗组与对照组相比明显升高 (P<0 .0 5 )。内毒素、肿瘤坏死因子、可溶性白细胞介素 2受体在内、外引流后 14d ,治疗组与对照组相比明显下降 (P<0 .Objective Therapeutical effect of recombinant human growth hormone (rhGH) on obstructive jaundice and internal and external drainage was observed.Methods New Zealand white rabbits were randomly divided into groups below: obstructive jaundice internal drainage plus rhGH group, obstructive jaundice internal drainage plus NS group, obstructive jaundice external drainage plus NS group, and obstructive jaundice external drainage plus rhGH group. After the establishment of obstructive jaundice model, rhGH was used in the above groups. Subcutaneous injection of rhGH 0.2 IU/kg was given twice a day. Isovolume NS was used on the control groups. Full set of endotoxin, tumor necrosis factor, sIL 2R and nutritional status were estimated before the model establishment, and 14 days after the model established, 14 days after internal and external drainage.Results Four days after internal and external drainage, body weight of therapy groups was increased compared with control groups ( P <0.05). Seven days and ten days after obstructive jaundice, blood sugar of therapy groups rised compared with control groups ( P <0.05). Albuminate, siderophilin and prealbumin of therapy groups were all observed an increase after 14 days after obstructive jaundice, and 14 days after internal and external drainage ( P <0.01). Blood total cholesterol, low density lipoprotein and omni bile acid of therapy groups after 14 days of obstructive jaundice were increased apparently ( P <0.05). Blood glutamic oxal acetic transaminase, transglutaminase, total bilirubin, blood uria nitrogen, creatinine and uric acid of therapy group after 14 obstructive jaundice days were increased ( P <0.05). Ca 2+ of therapy groups 14 days after obstructive jaundice, 14 days after internal and external drainage rised as compared with control groups ( P <0.05). However, K +,Na + of therapy groups 14 days after external drainage decreased ( P <0.05). An increasing tendency of sIL 2R was observed in control groups 14 days after obstructive jaun

关 键 词：梗阻性黄疸 重组人生长激素 内毒素 肿瘤坏死因子 可溶性白细胞介素2受体 

分 类 号：R657.43[医药卫生—外科学]