大鼠隐睾生殖细胞发育和凋亡与eNOS蛋白与mRNA表达  

Relationship of eNOS expression to the germ cell development and apoptosis in the rat cryptorchid

作　　者：郑航[1] 李世文[1] 郑新民[1] 胡礼泉[1] 王小芹[1] 

机构地区：[1]武汉大学中南医院武汉大学医学院泌尿外科男科学研究中心,430071

出　　处：《中华泌尿外科杂志》2002年第11期659-661,共3页Chinese Journal of Urology

基　　金：湖北省教委资助课题 (99A0 5 4)

摘　　要：目的　探讨内皮型一氧化氮合酶 (eNOS)蛋白及其mRNA表达与实验性大鼠隐睾生殖细胞发育和凋亡的关系。　方法　采用生物素 dUTP/酶标亲和素测定法检测SD大鼠单侧隐睾模型睾丸生殖细胞凋亡 ,免疫组化方法检测大鼠睾丸生殖细胞中eNOS基因表达 ,原位杂交法检测大鼠生殖细胞中eNOSmRNA的表达。　结果　术后第 7天隐睾侧睾丸发生凋亡的生殖细胞数与对侧正常睾丸相比显著增加 (P <0 .0 1)。术后第 4 0天 ,对侧正常睾丸的精子细胞中可见eNOS基因表达。术后第 3、7天 ,隐睾侧睾丸生殖细胞中eNOSmRNA表达与对侧正常睾丸相比无明显变化 (P >0 .0 5 )。　结论　在大鼠青春前期 ,eNOS基因表达与雄激素生成和生殖细胞发育有关 ;在成年期 ,eNOS基因表达可能仅与精子的成熟及活力有关而与生殖细胞凋亡无关。Objective To study the relationship of epithelial nitric oxide synthase (eNOS) expression with the germ cell development and apoptosis in the rat cryptorchid. Methods Germ cell apoptosis was detected by terminal deoxynucleotidyl transferase mediated d UTP nick end labeling (TUNEL) in the unilateral cryptorchid.Expression of eNOS was detected by immunohistochemical S P method. The dispersion characteristics of expression of eNOS mRNA was investigated by in situ hybridization. Results On the 7th day after the operation,as compared to the control,the number of apoptotic germ cell in the cryptorchid was increased significantly,but its testis weight was decreased predominantly,and expression of eNOS gene was localized to the cytoplasm of Legdig cell,Sertoli cell and germ cell in both testes.On the 40th day,expression of eNOS gene was localized to the cytoplasm of lengthened spermid.On the 3rd day and 7th day,expression of eNOS mRNA had no apparent change in the cryptorchid as compared to the control. Conclusions In adolescence,expression of eNOS regulates secretion of hormone and germ cell development;whereas after adulthood,expression of eNOS is only related to the mature and activity of sperm.Expression of eNOS has no apparent relationship with germ cell apoptosis.

关 键 词：大鼠 隐睾 生殖细胞发育 细胞凋亡 eNOS蛋白 MRNA 

分 类 号：R697[医药卫生—泌尿科学]