重组病毒法:一种快速检测HIV药物敏感性方法  

Recombinant Virus Assay:A Rapid Assay for Assessment of Drug Susceptibility of Human Immunodeficiency Virus Type 1 Isolates

作　　者：秦树林[1] Victor Arendt Robert Hemmer Francois Schneider 王爱霞[1] 盛瑞媛[1] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院感染内科,北京100730

出　　处：《中国医学科学院学报》2002年第5期523-526,共4页Acta Academiae Medicinae Sinicae

摘　　要：目的建立一种快速检测所有HIV病毒株药物敏感性的新方法(重组病毒法)。方法用逆转录-聚合酶链法(RT-PCR)扩增HIV/AIDS患者血浆中的HIVRNA,得到全长HIV逆转录酶(RT)的编码序列,然后与无感染性的前病毒克隆pHIV△RTBstEⅡ经过同源重组产生活的、具有诱导合胞体(SI)表型的重组HIV,再用HelaCD4+细胞蚀斑减少法检测HIV对核苷类药物的敏感性。结果用这种方法检测了7株不同表型的HIV,重组病毒与原始HIV病毒具有相同的基因型,药物敏感性结果与标准化外周血单个核细胞(PBMC)培养法的结果相同。结论重组病毒法是一种快速、准确检测所有表型HIV病毒株的药物敏感性方法。Objective To establish a rapid assay for assessment of drug susceptibility of human immunod-eficiency virus type1isolates (Recombinant virus assay).Methods This procedure allows the generation of viable virus with SI phenotype by homologous recombination of a RT-PCR-derived pool of reverse transcriptase (RT)coding sequences into an RT-deleted,noninfectious proviral clone,pHIV△RTBstEⅡ.Then the drug susceptibility of recombinant virus to RT inhibitors can be assessed in the Hela CD4 + plaque reduction assays.Results Analysis of7HIV strains with SI or NSI phenotype showed that recombinant viruses accurately exhibited the same genotype as that of the original HIV1isolates.The results of drug susceptibilities of HIV1isolate got by recombinant virus assay were the same as that by standardized peripheral blood mononuclear cell culture assay.Conclusion Recombinant virus assay is a rapid and accurate method to assess the drug sensitivity of HIV1isolates with SI or NSI phenotype.

关 键 词：重组病毒法 检测 药物敏感性 艾滋病毒 

分 类 号：R512.91[医药卫生—内科学]