自身反应性T细胞系及克隆的建立与TCR使用情况分析  

Generation and TCR usage analysis of autoreactive T cell line and clones from BXSB mice

作　　者：韩淑红[1] 陈蕴涛[1] 吕平[1] 朱晓辉[1] 张玲[1] 张岩[1] 高晓明[1] 

机构地区：[1]北京大学医学部免疫学系,100083

出　　处：《中华微生物学和免疫学杂志》2002年第6期595-598,共4页Chinese Journal of Microbiology and Immunology

摘　　要：目的　体外建立可长期生长的自身反应性T细胞系及克隆 ,并分析其T细胞受体的使用情况。方法　用狼疮小鼠BXSB自身脾脏作为刺激细胞 ,在体外建立长期生长的自身反应T细胞系(ATL) ,通过有限稀释法进行克隆 ,应用锚定PCR方法分析其特异的T细胞受体应用。结果　建立了H 2 b 限制性的自身反应性T细胞系和 2个T细胞克隆。T细胞系主要应用TRBV2S1、TRAV5S1和TRAV10S1,而 2个T细胞克隆均为TRBV2S1和TRAV10S1。分析其表型为CD3+ CD4 + CD2 8+ 。结论　从狼疮小鼠获得了自身反应性T细胞系及克隆 。Objective To establish long life span cultured autoreactive T cell line and clones and to analyze their T cell receptor usage. Methods Lymph node T cells from BXSB mice were repeatedly stimulated with syngeneic spleen cells to establish autoreactive T cell line. T cell clones were obtained by limiting dilution technique. T cell receptor usage was analyzed with Anchor PCR and Nested PCR; the phenotype was identified using FACS. Results We isolated, in the absence of a known stimulating antigen, a autoreactive CD4 + T cell line(ATL) and two T cell clones from BXSB mice. TCR TRBV2S1, TRAV5S1 and TRAV10S1 were used in ATL. Two T cell clones used TRBV2S1 and TRAV10S1. Its phenotype is mainly CD3 +CD4 +CD28 +. Conclusion The TCR usage of T cell line ATL and T cell clones is limited.

关 键 词：克隆 自身反应性T细胞 系统性红斑狼疮 有限稀释法 锚定PCR方法 动物模型 

分 类 号：R392[医药卫生—免疫学]