旋毛虫成虫可溶性抗原免疫小鼠攻击感染后的免疫应答  

Immune Responses to Challenge Infection in Mice Immunized with Trichinella spiralis Adult Worm Soluble Antigen

作　　者：申丽洁[1] 罗志勇[2] 朱声华[3] 

机构地区：[1]大理医学院寄生虫学教研室,大理671000 [2]大理医学院医学系,大理671000 [3]四川大学华西医学中心寄生虫学教研室,成都610044

出　　处：《中国寄生虫学与寄生虫病杂志》2002年第5期292-294,共3页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：目的　探讨旋毛虫成虫可溶性抗原 (AWSAg)接种小鼠后诱发的免疫应答。　 方法　制备旋毛虫AWSAg免疫小鼠 ,分别于攻击感染后 7、14、2 1、2 8和 3 5d ,动态观察免疫小鼠特异性IgG抗体水平、IL 2水平和T淋巴细胞亚群的变化。　结果　与非免疫鼠相比 ,免疫鼠血清特异性IgG抗体OD值在攻击感染后 7d明显升高 ,在观察期内一直高于非免疫鼠。感染后 7dIL 2水平明显增高 ,达观察期内最高值 ,2 1d后才逐渐减少 ,在感染后 2 8～ 3 5d仍高于正常组。免疫鼠CD4+ T细胞在攻击感染 7d明显增加并保持不变 ,非免疫鼠CD4+ T细胞及CD4/CD8比值明显较正常鼠减低。　结论　旋毛虫AWSAg免疫小鼠攻击感染后 ,出现细胞、体液免疫应答。Objective To study the immune response induced by the mice immunized with Trichinella spiralis(T.spiralis) adult worm soluble antigen(AWSAg). Methods T.spiralis AWSAg was prepared to immunize Kunming strain mice. Dynamic changes of IgG, IL 2 and T lymphocyte subsets from immunized mice were determined after challenge infection on d 7, d 14 , d 21 , d 28 and d 35 . Results On d 7 after challenge infection, IgG and CD4 + T cells of immunized group were markedly elevated and persisted higher over the observation period. In contrast, CD4 + T cells and CD4/CD8 ratio were significantly decreased in unimmunized group resulted from immune suppression after infection. IL 2 levels reached the peak on d 7 and persisted in high level from d 7-d 21 in both immunized and unimmunized group after infection, then decreased gradually. Till 35 days after infection, IL 2 level was still higher than the normal mice. Conclusion Mice immunized with AWSAg of T. spiralis produced a potential cellular and humoral immune response.

关 键 词：旋毛虫 成虫可溶性抗原 保护性免疫 T淋巴细胞亚群 旋毛虫免疫 AWSAg 

分 类 号：R532.14[医药卫生—内科学]