伯氏疟原虫氯喹抗性株和敏感株基因组DNA比较研究  

Comparative Study on Genomic DNA of Malaria Parasite: Chloroquine-resistant and Chloroquine-sensitive Strains of Plasmodium berghei

作　　者：陈克强[1] 宋关鸿[1] 朱淮民[1] 苏新专 

机构地区：[1]第二军医大学病原生物学教研室 [2]Lab.of Parasitic Disease, National Institute of Health, Bethesda

出　　处：《中国寄生虫学与寄生虫病杂志》2002年第5期295-297,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国家自然科学基金 (No .39880 0 4 1 )~~

摘　　要：目的　比较伯氏疟原虫敏感株 (N株 )和抗性株 (RC株 )基因组DNA差异。　方法　应用基因组DNA随机多态性扩增 (RAPD)和锚定引物扩增DNA(APAD) ,分别对抽提的伯氏疟原虫N株和RC株基因组DNA进行PCR扩增 ,并对扩增的产物进行电泳 ,对结果进行统计分析。　结果　 3 7条随机引物RAPD方法扩增出 44 0条DNA条带 ,其中RC株和N株的共有条带是 196条 ,差异带 43条。 84条锚定多聚A引物APAD方法扩增出 95 2条DNA条带 ,其中RC株和N株的共有条带是 43 6条 ,差异带 5 3条。两种方法得到N株和RC株基因组DNA的同源性分别为 0 .89和0 .91;距离系数分别为 0 .197和 0 .15 5。　结论　伯氏疟原虫N株和RC株基因组DNA具有高度同源性。Objective To investigate the genomic DNA difference between chloroquine sensitive (N) and chloroquine resistant (RC) strains of Plasmodium berghei . Methods By means of polymerase chain reaction(PCR), the abstracted genomic DNAs of the N and the RC strains of Plasmodium berghei were amplified with 37 random primers(RAPD) and 84 anchored primers (APAD). The PCR products were analysed by electrophoresis and statistics. Results Of 440 bands amplified with RAPD, 196 bands were common to both strains whereas 43 bands were unique to either the RC or the N strain, with a similarity coefficient (SI) of 0.89, and a distance coefficient (D) of 0.197. Similarly, among 952 bands amplified with APAD, 436 bands were shared by both strains, and 53 bands were unique to either of them with SI 0.91 and D 0.155 respectively. Conclusion The genomic DNAs between the RC and the N strains of Plasmodium berghei are highly homogeneous.

关 键 词：伯氏疟原虫 氯喹抗性株 RAPD APAD 基因组DNA 抗药性 致病力 

分 类 号：R382.31[医药卫生—医学寄生虫学]