马来丝虫长爪沙鼠动物模型保种衰退期的观察  

Observation on Decline Phase of Maintenance of Brugia malayiMeriones unguiculatus Model

作　　者：陈韶红[1] 孙德建[1] 施恒华[1] 袁以真[1] 

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所

出　　处：《中国寄生虫学与寄生虫病杂志》2002年第5期308-309,共2页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：目的　观察马来丝虫经长爪沙鼠传代的衰退情况。　方法　用马来丝虫微丝蚴感染中华按蚊 ,收集感染期幼虫 (L3) ,通过腹腔接种感染长爪沙鼠 ,连续观察 3 3代长爪沙鼠体内马来丝虫微丝蚴的发育情况。　结果　从 1974年建立的马来丝虫长爪沙鼠动物模型至今 ,通过 3 3代传代 ,发现随着转种代数增加 ,从第 2 8代起长瓜沙鼠的阳性率逐年下降 ,由 2 8代的 80 %下降至 3 2代的 16% ,到 3 3代时阳性率降为 0。　结论　经过较长期的传代 ,马来丝虫幼虫难以在长爪沙鼠体内发育繁殖。Objective To observe the degeneration of Brugia malayi in Meriones unguiculatus model. Methods Microfilaria of Brugia malayi derived from Meriones unguiculatus was used to infect Anopheles sinensis . Infective stage larvae (L 3) from mosquito vector were collected and inoculated into abdomen of Meriones unguiculatus. Successive 33 generations of the parasite in the rodent model have been observed. Results Since 1974 when the animal model was established, the parasite has lasted for 33 generations, the positive rate of Meriones unguiculatus with microfilaria gradually reduced from 80% of the 28th generation to 16% of the 32nd generation and finally to 0 at the 33rd generation. Conclusion It became difficult for the larvae of Brugia malayi to develop and/or reproduce in the animal model after multiple inoculations for generations.

关 键 词：马来丝虫 动物模型 保种衰退期 传代 

分 类 号：R383.16[医药卫生—医学寄生虫学]