增生性瘢痕胶原降解与形态学的观察  

An observation of the morphology and the degradation of hypertrophic scar collagen

作　　者：武继祥[1] 魏喜全[2] 吴宗耀[1] 李世荣[1] 

机构地区：[1]第三军医大学西南医院康复整形中心,重庆400038 [2]第三军医大学门诊部

出　　处：《中华烧伤杂志》2002年第5期296-298,共3页Chinese Journal of Burns

摘　　要：目的　探讨胶原降解和瘢痕形态结构在增生性瘢痕形成中的作用。方法　应用SDS 聚丙烯酰胺凝胶加胶原底物电泳、氨基酸分析法和复合染色法 ,观测增生性瘢痕组织中胶原酶活性、胶原降解和瘢痕的组织形态。　结果　增生性瘢痕组织胶原纤维排列紊乱 ,胶原纤维间有大量的酸性粘多糖 ,紧密包绕胶原纤维 ,胶原酶活性明显下降 ,胶原降解减少。　结论　胶原酶活性降低 。Objective To explore the role of collagen degradation and scar morphology and structure in the formation of hypertrophic scar. Methods SDS PAGE(sodium dodecyl sulfate polyacrylamide gel electrophoresis) plus collagen substrate electrophoresis, amino acid analysis and compound staining were employed to observe the collagenase activity within hypertrophic scar, collagen degradation and the tissue morphology of the scar. Results There exhibited deranged collagen fibres within hypertrophic scar, and large amounts of acid mucopolysaccharide closely surrounded the collagen fibres. All these led to an obvious decrease in collagenase activity and reduction of collagen degradation. Conclusion The decrease of collagen degradation and the formation of hypertrophic scar might be closely related to the decrease in collagenase activity and the inhibiting activity of acid mucopolysaccharide on collagenase. [

关 键 词：增生性瘢痕 胶原酶 胶原降解 组织形态学 酸性粘多糖 

分 类 号：R622[医药卫生—整形外科]