献血者体内携带的HCV包膜区基因同源性分析  

Homology analysis on HCV envelope gene of carriers from blood donors

作　　者：王迅[1] 郑岚[1] 许莉萍[1] 刘达庄[1] 高峰[1] 

机构地区：[1]上海市血液中心,200051

出　　处：《临床输血与检验》2002年第4期15-17,共3页Journal of Clinical Transfusion and Laboratory Medicine

摘　　要：目的　建立 HCV包膜区基因同源性分析方法 ,探讨献血者体内携带的 HCV包膜区基因变异情况。　方法　通过 RT-PCR和序列测定 ,对 9例献血者体内携带的 HCV基因组中高变的包膜区 (E1和 E2 / NS1区 )基因变异进行了横断面的同源性分析。　结果　不同型 HCV毒株间的同源性只有 5 6.99%～ 68.80 % ;同一亚型内毒株间的同源性为80 .3 3 %～ 96.40 % ,且有地区性分布的特点。　结论　在无症状的 HCV携带献血者中 ,不同 HCV毒株间高变区基因的变异相当大 ,以此可在分子水平上对丙型肝炎的传播途径进行确认 ,对供受者间输血后丙型肝炎的因果关系进行鉴定。Objective To establish homology analysis method on HCV envelope gene and find out the variation of HCV envelope gene of carriers from blood donors. Methods RT PCR and sequence analysis were used to study the transversal homology on HCV envelope gene fragments (E1 and E2/NS1) in 9 carriers. Results\ The homology of HCV envelope gene between different types is 56 99%～68 80%,and 80 33%～96 40% between the same subtypes.It exists geographic distribution on HCV subtypes. Conclusion\ The variation of different HCV strains in HVR fragment is great between carriers from blood donors without symptoms.This character can be used in trailing the transmitted pathway of hepatitis C at the molecular level,and determine the causality of post transfusion hepatitis C between the donors and the recipients.

关 键 词：丙型肝炎病毒 包膜区 变异性 基因型 输血安全 RT—PCR 

分 类 号：R373.21[医药卫生—病原生物学] R457[医药卫生—基础医学]