测定人血浆中辅酶Q_(10)的反相高效液相色谱法  

Determination of Coenzyme Q_(10) in Human Plasma by Reverse-phase High-performance Liquid Chromatography

作　　者：马金才[1] 宋秀红 

机构地区：[1]军事医学科学院卫生学环境医学研究所,天津300050 [2]沈阳军区联勤部卫生防疫队,辽宁沈阳110031

出　　处：《分析测试学报》2002年第6期89-91,共3页Journal of Instrumental Analysis

摘　　要：用反相高效液相色谱法测定了人血浆中的辅酶Q10,样品用正己烷萃取 ,甲醇 -乙醇 (体积比70∶30,含20mmol/LLiClO4)为流动相 ,在WatersμBondapakC18 柱上分离并于波长275nm下检测 ,外标法定量 ;方法线性范围为10～100ng,r=0.9998,平均回收率为96 % ,相对标准偏差为3.0 % ;方法检出限为2ng(质量 )和0.2mg/L(质量浓度 )。Reverse - phase high - performance liquid chromatography with UV detector was investigated as a method for the assay of coenzyme Q10 in human plasma. After deproteinization sample was extracted by n - hexane. Separation was performed in a Waters μBondapak C18 column at 25 ℃ by using an eluent of methanol-ethanol(70∶30 by volume, including 20 mmol/L LiClO4) with flow rate of 1.0 mL/min, the eluate was monitored by UV detector at 275 nm. Coenzyme Q10 was quantitatively determined by an external standard method. Detection limit of 2 ng and 0.2 mg/L were achieved. A linear range of 10～100 ng, correlation coefficient of 0.999 8, RSD of 3.0%, and average recovery of 96%, were obtained for the method.

关 键 词：测定 人血浆 反相高效液相色谱 辅酶Q10 心血管疾病 临床检验 

分 类 号：R54[医药卫生—心血管疾病] R446.11[医药卫生—内科学]