溶脲脲原体感染对大鼠Sertoli细胞分泌IL-6和TGF-β_1的影响  

The effect of Ureaplasma Urealyticum infection on the IL-6 and TGF-β_1 secretion by rat Sertoli cells

作　　者：王保国[1] 席晔斌[1] 陈广洁[1] 包士三[2] 李伟毅[1] 

机构地区：[1]上海第二医科大学上海免疫学研究所,上海200025 [2]澳大利亚悉尼大学病理系

出　　处：《中国免疫学杂志》2002年第11期783-786,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金资助项目 (批准号 3 9970 2 83 )

摘　　要：目的 :研究大鼠Sertoli细胞在感染中的免疫调节作用。方法 :SD大鼠的睾丸经Ⅱ型胶原酶和透明质酸酶二步消化、过滤、离心获得高纯度、高活率的Sertoli细胞。体外培养的Sertoli细胞经溶脲脲原体 (UU)、UU上清和热灭活UU感染或作用 ,用ELISA法分析、观察UU感染时Sertoli细胞分泌IL 6和TGF β1 的变化。结果 :低剂量的活UU和UU上清能明显上调Ser toli细胞分泌IL 6的功能 (P <0 0 1) ,而高剂量时则表现为明显的抑制作用 (P <0 0 1) ;低、高剂量的活UU、UU上清和热灭活UU均能明显抑制Sertoli细胞分泌TGF β1 的功能 (P <0 0 1)。结论 :大鼠Sertoli细胞在睾丸局部感染时通过其分泌的IL 6和TGF β1 可发挥免疫调节作用。Objective:To investigate the role of immune regulation of the rat Sertoli cells in the infection Methods:The testis of the SD rat was digested by Collagenase (typeⅡ) and hyaluronidase, after filtrating and centrifugalizing, the rat Sertoli cells (higher purity) were obtained After Sertoli cells (in vitro) were infected by living Ureaplasma Urealyticum (UU)?supernatant without UU and heat inactivated UU, the effect of UU infection on the IL 6 and TGF β 1 secretion by rat Sertoli cells were analysed by ELISA methods Results:The living UU and the supernatant without UU, in low dose, increased the IL 6 secretion by rat Sertoli cells (P<0 01); but in high dose, it inhibited the IL 6 secretion (P<0 01) Meanwhile, living UU?UU supernatant and heat inactivated UU, in low and high dose, inhibited the TGF β 1 secretion by rat Sertoli cells (P<0 01) Conclusion:Sertoli cell can play the role of immune regulation, with the secretion of the IL 6 and TGF β 1, in the infection of testis by UU in rat.

关 键 词：溶脲脲原体感染 大鼠 SERTOLI细胞 IL-6 TGF-β1 泌尿生殖道感染 

分 类 号：R691.3[医药卫生—泌尿科学] R392.[医药卫生—外科学]