鸡贫血病毒细胞凋亡素基因的原核表达及其免疫学活性  

Expression of Chicken Anaemia Virus(CAV) Apoptin Gene in E.coli and Analysis of It′s Immunity

作　　者：刘岳龙[1] 秦爱建[1] 金文杰[1] 叶建强[1] 吉荣[1] 崔治中[1] 段玉友[1] 

机构地区：[1]扬州大学畜牧兽医学院,江苏扬州225009

出　　处：《中国兽医学报》2002年第6期541-543,共3页Chinese Journal of Veterinary Science

基　　金：江苏省自然科学基金资助项目 ( BJ930 94) ;扬州大学科研基金资助项目 ( 35 31)

摘　　要：将鸡贫血病毒 (CAV)细胞凋亡素基因克隆入表达性载体 p GEX- 5 X- 3,在大肠杆菌中以融合蛋白的形式获得表达。再以表达产物免疫小鼠 ,制备抗 CAV凋亡素的多克隆抗体。以其对 CAV感染的 MSB1细胞作间接免疫荧光试验(IFA)检测呈阳性 ,表明表达产物保留了其相关的天然抗原特性。CAV apoptin gene was inserted into pGEX-5X-3 vector,then expressed by in E.coli in a fusion protein form.The anti-CAV polyvalent antibody was produced from mouse immunized with the fusion protein,and its specificity was detected positively on CAV-infected MSB1 cells by indirect immunoinfluscent assay(IFA).This results showed the expressed protein retained some antigenicities of the native CAV apoptin.

关 键 词：细胞凋亡素 原核表达 免疫学活性 鸡贫血病毒 间接免疫荧光试验 基因克隆 

分 类 号：S852.659.2[农业科学—基础兽医学]