影响"扬稻6号"成熟胚愈伤组织再生植株的因子  

Factors Affecting Plant Regeneration from Callus of Mature Embryo of "Yangdao 6

作　　者：佘建明[1] 张保龙[1] 倪万潮[1] 

机构地区：[1]江苏省农业科学院农业生物遗传生理研究所,江苏南京210014

出　　处：《江苏农业学报》2002年第4期199-202,共4页Jiangsu Journal of Agricultural Sciences

基　　金：国家科技部"863"计划重大项目(Z17 01 01)

摘　　要：　以水稻中籼品种"扬稻6号"的成熟胚为外植体材料诱导愈伤组织发生。在愈伤组织继代培养阶段,研究山梨醇、甘露醇、脱落酸、蔗糖和麦芽糖对愈伤组织分化植株能力的影响作用,并探讨在愈伤组织诱导阶段添加6 苄基氨基嘌呤对后期愈伤组织分化植株能力的影响程度。试验结果表明,在愈伤组织继代培养基中添加30g/L山梨醇,3～5mg/L脱落酸和在愈伤组织诱导培养基中附加0.2mg/L6 苄基氨基嘌呤有利于建立起较高频率的植株再生体系,愈伤组织经过继代培养后其植株再生的频率保持在50%左右。Calli were induced from mature embryoes of medium indica rice variety'Yangdao 6'. Effects of sorbitol, mannitol, absciss acid, sucrose and malt sugar on plant regeneration were studied during callus subcultures. At the stage of callus induction, influence of 6Benzylaminopurine on plant regeneration was also surveyed. Results indicated that 30 g/L sorbitol, 3～5 mg/L absciss acid added to subculture medium and 0.2 mg/L 6Benzylaminopurine added to induction medium were advantageous for establishing the plant regeneration system with higher frequency. After callus subculture, the frequency of plant regeneration from calli maintained about 50%.

关 键 词：扬稻6号 成熟胚 愈伤组织 再生植株 籼稻 

分 类 号：S511.21[农业科学—作物学]