黄芪多种成分抗人疱疹病毒的初步实验研究  

The experimental research of antiherpes simplex virus effects of various components in the root of Astragalus membranaceus

作　　者：王志洁[1] 黄铁牛[2] 刘焱文[3] 鲁遂荣[1] 杨占秋[1] 方学韫[1] 

机构地区：[1]武汉大学医学院病毒研究所,武汉430071 [2]中南财经政法大学 [3]湖北中医学院中药系

出　　处：《中国现代应用药学》2002年第5期356-359,共4页Chinese Journal of Modern Applied Pharmacy

基　　金：湖北省自然科学基金资助

摘　　要：目的 :为了开发利用黄芪的抗病毒性质 ,我们进行了此项研究。方法 :我们以阿昔洛韦 (ACV)为阳性对照 ,采用对病毒所致细胞病变的抑制及空斑减数实验 ,观察了黄芪总皂苷、总多糖、总黄酮抗HSV药效。结果 :在Hep - 2细胞系统中 ,ACV对HSV - 1HS - 1株直接杀灭、感染阻断、增殖抑制的ED5 0为 30 .83ug/ml,16 .0 4ug/ml,2 0 .0 4ug/ml;对HSV - 2 333株的相应ED5 0为 16 .45ug/ml,18.6 2ug/ml,10 .85ug/ml.黄芪总皂苷对HSV - 1HS - 1株相应ED5 0为 1.6 8ug/ml,1.72ug/ml,1.95ug/ml;对HSV - 2 333株相应ED5 0为 1.73ug/ml,2 .70ug/ml,2 .74ug/ml.黄芪总多糖对HSV - 1HS - 1株的相应ED5 0为4.0 3ug/ml,5 .33ug/ml,4.90ug/ml;对HSV - 2 333株相应ED5 0为 6 .0 4ug/ml,5 .43ug/ml,7.5 0ug/ml.黄芪总黄酮对HSV - 1HS - 1株的相应ED5 0为 4.95ug/ml,2 .75ug/ml,3.49ug/ml;对HSV - 2 333株的相应ED5 0为 3.5 6ug/ml,3.93ug/ml,6 .2 7ug/m。 结论 :黄芪总皂苷对HSV - 1的治疗指数 (TI)为ACV的 4.4,2 .4,2 .5倍 ;对HSV - 2的治疗指数为ACV的 2 .3,1.0 ,1.6倍。总多糖对HSV - 1的治疗指数为ACV的 10 .3,4.1,5 .5倍 ;对HSV - 2的治疗指数为ACV的 3.7,2 .7,3.3倍。总黄酮的治疗指数与ACV相近。OBJECTIVE:In order to understand and exploit the antiviral properties of Astragalus membranaceus. METHOD:ACV was used as positive control of antiherpes,through inhibitions of cellular pathological effects of herpes simplex virus type-2 and plaque reduction assays,the effects of antiherpes of total saponins ,total polysaccharieds,total flavonoids of Astragalus membranaceus were observed. RESULTS:In Hep-2 cell systems,the median effect doses(ED50) of ACV of direct virucidal activity,the inhibition effects on the infection and multiplication of HSV-1 HS-1 strain were 30.83ug/ml,16.04ug/ml and 20.04ug/ml, respectively the corresponding figures to HSV-2 333 strain were 16.45ug/ml,18.62ug/ml and 10.85ug/ml, respectively; the corresponding ED50s of total saponins to HSV-1 HS-1 strain were 1.68ug/ml,1.72ug/ml and 1.95ug/ml,respectively; those to HSV-2 333 strain were 1.73ug/ml,2.70ug/ml and 2.74ug/ml, respectively; the corresponding ED50s of total polysaccharides to HSV-1 HS-1 strain were 4.03ug/ml,5.33ug/ml and 4.90ug/ml, respectively; those to HSV-2 333 strain were 6.04ug/ml,5.43ug/ml and 7.50ug/ml, respectively.The corresponding ED50s of total flavonoids to HSV-1 HS-1 strain were 4.95ug/ml,2.75ug/ml and 3.49ug/ml, respectively; to HSV-2 333 strain were 3.56ug/ml,3.93ug/ml and 6.27ug/ml, respectively. CONCLUSION:The Treatment Indices(TI) of total saponins to HSV-1 HS-1 strain were 4.4,2.4 and 2.5times of ACVTIs,to HSV-2 333 strain were 2.3,1.0 and 1.6 times of ACVTIs.The TIs of total polysaccharides to HSV-1 HS-1 strain were 10.3,4.1 and 5.5 times of ACVTIs;to HSV-2 333 strain were 3.7,2.7 and 3.3 times of ACVTIs.The TIs of total flavonoids were close to ACVTIs.These components of Astragalus membranaceus should be worth exploitation.

关 键 词：黄芪 实验研究 黄芪总皂苷 黄芪总多糖 黄芪总黄酮 抗病毒 人疱疹病毒 

分 类 号：R282.71[医药卫生—中药学] R285[医药卫生—中医学]