良园枇杷膏对豚鼠气道收缩和炎症反应的作用  

Effect of Liangyuan Pipagao on brochoconstriction and inflammation in the sensitized guinea pig

作　　者：谢强敏[1] 沈文会[1] 陈季强[1] 杨秋火[1] 卞如濂[1] 

机构地区：[1]浙江大学医学院国家药品监督管理局浙江呼吸药物研究实验室,杭州310031

出　　处：《中国现代应用药学》2002年第5期365-367,共3页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的 :良园枇杷膏 (下简称枇杷膏 )是一个传统的镇咳祛痰复方中成药。本文旨在研究其对气道平滑肌的扩张和抗肺部过敏性炎症的作用。方法 :采用肺功能测定法、离体气管法观察枇杷膏的支气管扩张和抗过敏作用 ;支气管肺泡灌流法观察抗炎作用。结果 :在整体试验中 ,枇杷膏 7.5 g/kg和 15 .0g/kgi.g抑制致敏豚鼠抗原攻击后引起的肺阻力 (RL)增加和动态肺顺应性 (Cdyn)降低 ;抑制支气管肺泡灌流中嗜酸性粒细胞增加 ,ID50 (95 %可信限 )为 1.98(0 .41～ 9.6 4)g/kg ;在离体试验中 ,枇杷膏呈剂量依赖反应松弛豚鼠离体气管平滑肌 ,IC50 (95 %可信限 )为 1.2 3(0 .97～ 1.5 4)× 10 4 g .L 1;抑制Schultz Dale反应 ,ID50 (95 %可信限 )为 4.5 5 (2 .78～ 7.42 )× 10 5g .L 1。结论OBJECTIVE:Liangyuan Pipagao ( LYPPG )is a compound preparation of traditional Chinese medicine applied to antitussive and expectorant. However, the studies evaluated effect of the drug on brochoconstriction and inflammation in the sensitized guinea pig. METHOD:pulmonary mechanics changes in the ovalbumin-sensitized guniea pigs induced by ovalbumin aerosol in vivo, Schultz-Dale reaction of the ovalbumin-sensitized trachea and isolated resting tracheal relaxing in guinea pigs in vitro were investigated. RESULTS:LYPPG 7.5 g/kg and 15.0 g/kg by ig remarkably prevented the alteration of lung resistance( R L ) and dynamic lung compliance ( C dyn ) induced by antigen challenge, and 0.83g/kg,2.5g/kg and 7.5g/kg by ig inhibited antigen-induced inerense of eosinophils in the bronchoalveolar lavage fluid of the sensitized guinea-pigs in vivo, ID 50 value (95% confidence limits)= 1.98(0.41～9.64)g/kg. LYPPG relaxed the rest isolated tracheal smooth muscle by dosage, IC 50 (95% confidence limits)= 1.23 (0.97～1.54)×10 -4g.L -1,and inhibited Schultz-Dale reaction in guinea pigs in vitro, ID 50 (95% confidence limits)= 4.55(2.78～7.42)×10 -5g.L -1. CONCLUSION:These results indicated that LYPPG had a significant pharmacological effects on bronchodilatation and anti-allergy inflammation.

关 键 词：良园枇杷膏 豚鼠 气道收缩 炎症反应 肺阻力 动态肺顺应性 嗜酸性粒细胞 哮喘 

分 类 号：R285.5[医药卫生—中药学] R259.[医药卫生—中医学]