类风湿关节炎继发肺间质病变患者激素冲击治疗前后CD8^+CD28^-T细胞的变化及意义  

Expression and significance of CD8^+CD28^-T cells for patients with interstitial lung disease secondary to rheumatoid arthritis who underwent methylprednisolone impact therapy

作　　者：吴卫锋[1] 吴洁文[2] 邢窕思 

机构地区：[1]揭阳市人民医院呼吸内科,广东揭阳522000 [2]汕头大学医学院第一附属医院呼吸内科,广东汕头515000 [3]美国东卡罗莱纳大学布罗迪医学院解剖与生物细胞学系,美国东卡罗莱纳州27834

出　　处：《分子影像学杂志》2015年第3期246-248,共3页Journal of Molecular Imaging

摘　　要：目的探讨类风湿关节炎(RA)继发肺间质病变(ILD)患者激素冲击治疗前后CD8+CD28-T细胞的变化与意义。方法共入选23例RA-ILD患者(A组),选取同期就诊的RA患者25例(B组)、非RA致ILD患者25例(C组)、25例健康人(D组)作为对照。对以上具有甲泼尼龙冲击适应症的A组患者进行治疗,治疗前后采用流式细胞术检测上述4组外周血CD8+CD28-T细胞及其细胞因子白介素10(IL-10)和转化生长因子β1(TGF-β1)的百分含量变化。结果与D组比较,A、B及C三组的CD8+CD28-T细胞、IL-10及TGF-β1均明显升高,含量由高至低均表现为A>C>B>D,且四组间任意两组上述三项指标均有统计学差异(P<0.05)。治疗后A组患者CD8+CD28-T细胞、IL-10及TGF-β1均较治疗前明显降(P<0.05)。结论 CD8+CD28-T细胞在类风湿关节炎继发肺间质病变发病过程可能起到重要作用,而甲泼尼龙可显著抑制该细胞活性,有望作为结缔组织病致肺间质病变的病情观察及疗效评价指标。Objective To investigate the rheumatoid arthritis(RA) patients with secondary pulmonary interstitial disease(ILD)hormone impact CD8+CD28-T cells before and after the treatment with the change of meaning.Methods Included in the total of 23 patients with RA-ILD(group A),selection of RA patients during the same period of 25 cases(group B),the RA patients with ILD 25 cases(group C),25 cases of healthy people(group D) as A control.For more than A group of patients with A prednisolone impact indications for treatment,using flow cytometry to detect the above four groups before and after the treatment of peripheral blood CD8+CD28-T cells and cytokines interleukin 10(IL-10),and transforming growth factor beta 1(TGF-beta 1) the percentage of change.Results Compared with group D,A,B and C three groups of CD8+CD28-T cells,IL-10 and TGF-beta 1 were significantly increased,content from high to low were characterized by A>C>B>D,and four groups between any two sets of the above three indicators are statistically significant(P<0.05).A group of patients after treatment of CD8+CD28-T cells,IL-10 and TGF-beta 1 than before treatment significantly reduced(P<0.05).Conclusion CD8+CD28-T cells on the pathogenesis of rheumatoid arthritis secondary pulmonary interstitial lesions process may play an important role,and methyl prednisolone can significantly inhibit the cell activity,is expected to be as a connective tissue disease caused by pulmonary interstitial lesions was observed and curative effect evaluation index.

关 键 词：类风湿关节炎 继发肺间质病变 激素冲击治疗 CD8+CD28-T细胞 细胞因子 

分 类 号：R593.22[医药卫生—内科学]