核心抗体阴性乙型肝炎病毒感染者的核心基因分析  

THE CORE GENE OF AN HBsAg POSITIVE BUT ANTI-CORE ANTIBODY NEGATIVE SERUM

作　　者：卫清[1] 闻玉梅[1] 何丽芳[1] 李雪萍[1] 俞翠株 

机构地区：[1]上海医科大学微生物教研室 [2]上海市医学化验所

出　　处：《病毒学报》1992年第1期90-92,共3页Chinese Journal of Virology

摘　　要：1987年首次报道,在塞内加尔存在血清乙型肝炎病毒表面抗原(HBsAg)阳性,但核心抗体(anti-HBc)阴性的感染者。其血清中有直径为45～60nm的病毒,也有22～30nm直径的HBsAg颗粒。与常规乙型肝炎病毒(HBV)DNA探针进行杂交,15份感染者中仅有一份出现杂交微弱阳性,当时被称为是乙型肝炎病毒2型(HBV-2)。此后,法国、To study the core gene of Hepatitis B virus type 2 strain, an HBsAg positive but anti-core, anti-e and anti-HBs negative serum ( #61) was used to extract DNA. Primers were used to amplify partial Pre-core and core gene by polymerase chain reaction. The amplified product was cloned in vector pGEM 3Z, and a clone(CL 61-8)was further sequenced. By comparing the sequence to that of a preiously cloned conventional HBV DNA sequence(pADR-l),only 4 nucleotide variations were found. However, all variations did not lead to alterations in amino acid sequence. Our data do not support the former presumption that there are variations in the core gene and core protein in HBV-2, which is responsible for non-detectable anti-HBc.Therefore it is hi.ghly possible that lack of anti-HBc is due to defect in host immune responses, rather than variations in the virus perse.Complete immunetolerance versus HBc Ag may be the cause of non-detectable anti-HBc antibody.

关 键 词：乙肝病毒 核心基因 核心抗体 

分 类 号：R512.620.3[医药卫生—内科学]