补体介导的流行性出血热疫苗中和抗体的检测  

DETECTION OF COMPLEMENT-MEDIATED NEUTRALIZING ANTIBODY INDUCED BY INACTIVATED VACCINES AGAINST HEMORRHAGIC FEVER WITH RENAL SYNDROME( HFRS )

作　　者：姚智慧[1] 俞永新[1] 

机构地区：[1]中国药品生物制品检定所

出　　处：《病毒学报》1992年第2期148-151,共4页Chinese Journal of Virology

摘　　要：用空斑减少中和试验(Plaque reduction neutralization test,PRNT)和补体介导(Complement-mediated)的空斑减少中和试验(PRCNT)同时检测流行性出血热疫苗兔及人免疫血清的中和抗体,发现PRCNT不但可提高兔及人血清抗体的阳性检出率,测出中和抗体的滴度增加2～4倍,并且可扩大两型病毒间的交叉中和作用。提示流行性出血热疫苗免疫的兔和人血清中和抗体均有补体依赖的特性。A comparison of HFRS vaccine-induced neutralizing antibody assesed by conventional plaque reduction neutralization test ( PRNT ) and by complement-mediated PRNT ( PRCNT ) was studied. Increased serocoversion rates of sera in rats & humans vaccinated with various HFRS inactivated vaccine were found when PRNT was performed in the presence of complement. The neutralizing titers were shown to be enhanced by 2-4 fold. In the presence of complement, the cross-neutralization activity between type 1 & type 2 viruses was enhanced as well. These results show that HFRS vaccine-induced antibody in rabbit and human sera is complement-dependent .

关 键 词：补体 抗体 疫苗 流行性出血热 

分 类 号：R392－33[医药卫生—免疫学]