我国戊型肝炎病毒基因组cDNA全序列测定及分析  

MOLECULAR CLONING AND SEQUENCING OF THE WHOLE CHINESE HEPATITIS E VIRUS GENOME

作　　者：毕胜利[1] 刘崇柏[1] 曹学义[2] 赵洪兰[1] H.S.Margolis 

机构地区：[1]中国预防医学科学院病毒学研究所 [2]新疆维吾尔自治区卫生防疫站 [3]美国疾病控制中心肝炎实验室

出　　处：《病毒学报》1992年第3期271-279,共9页Chinese Journal of Virology

摘　　要：戊型肝炎最早在印度发现,随后相继在东南亚、中亚细亚、北非、墨西哥和中国等发生流行。1986～1987年在新疆南部大流行期间,采集急性黄疸型非甲非乙型肝炎患者的急性期血液和粪便,其中1份粪便标本(男性维吾尔族患者)经免疫电镜及免疫酶法(EIA)检测,证实含有直径27～34nm的廿面体病毒颗粒。采集的戊型肝炎患者急性期血清,用感染戊型肝炎病毒(HEV)的食蟹猴肝切片荧光染色证明抗-HEV IgM阳性。A solid phase antibody capture method has been developed to concen trat and purify the HEV particles from fecal samples. The samples were collected from an acute phase HEV patient during the epidemic of 1986 1987, in Xinjiang Ugur Autonomous Region, China.A total of 7.4kb of full-length HEV cDNA clone was generated and sequenced by Dideoxy chain termination method. There are 94% homology in nucleotides when compared with the genome of HEV cloned originally from a Burma HEV strain. The constituent ratio of the Chinese HEV genome was composed of 17.1% adenine, 24.9% thymine, 32.1% cytosine, and 25.9% guanine. The C + G content was 58%.

关 键 词：聚合酶链反应 戊肝病毒 

分 类 号：R373.21[医药卫生—病原生物学]