脾虚模型大鼠延脑腹外侧核小泡乙酰胆碱转运体免疫组化研究  

Vacht Immunohistoehemistry study on modal rat of chinese medical spleen deficience on nucleus ventralis latertis in medulla oblongata

作　　者：张晓杰[1] 

机构地区：[1]黑龙江省齐齐哈尔医学院病理教研室,黑龙江161042

出　　处：《医学研究通讯》2002年第11期36-37,共2页Bulletin of Medical Research

基　　金：黑龙江省科学基金

摘　　要：目的探讨脾虚模型大鼠胆碱能神经的变化。方法选用特异性较强小泡乙酰胆碱转运体(VAChT)抗血清,从形态学上观察模型大鼠胆碱能神经在延脑腹外侧核中的变化。结果模型大鼠延脑腹外侧核VAChT免疫反应与对照组相比明显减弱。结论脾虚大鼠胆碱能神经VAChT免疫反应明显减弱,VAChT是一种新的独特的研究中枢及外周胆碱能神经元的最有效标志物。Objective To study the changesr of cholinergic neurons in model rat with Chinese medical spleen deficience . Methods We selected very peculiar VAChT antibody marked microscopy observe to find the changes of cholinergic neurons of model animal' s nucleus ventralis lateralis (NVL) in medulla oblongata. Results VAChT immunohisto-chemistry reaction lightened obviously in cholinergic neurons of model animals nucleus ventralis lateralis ( NVL) in medulla oblongata, compared with normal group. Conclusion cholinergic neurons VAChT immunohistochemistry reaction decreased obviously in rats with Chinese medical spleen deficience. VAChT is a new and special marker for chohnergic neurosn in the central and peripheral neurol system.

关 键 词：脾虚 免疫组化 胆碱能神经 延脑 腹外侧核 VACHT 动物模型 免疫反应 

分 类 号：R256.3[医药卫生—中医内科学]