HPLC法测定醒脑滴鼻液中β-细辛醚、α-细辛醚的含量  

Determination of β-Asarone and α-Asarone in Xing Nao Nasal Drops by HPLC

作　　者：柯雪红[1] 魏刚[1] 方永奇[1] 林双峰[1] 

机构地区：[1]广州中医药大学第一附属医院实验中心,广州510405

出　　处：《中药新药与临床药理》2002年第6期389-390,共2页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广东省中医药管理局课题(编号97189)。

摘　　要：目的测定醒脑滴鼻液中β-细辛醚、α-细辛醚的含量。方法色谱条件:HypersilDivisionODSC18分析柱(150mm×4.6mm,5μm),流动相:甲醇-水=6∶4(1000mL中加入磷酸二氢钾1.4g、十二烷基磺酸钠1.2g);检测波长:257nm,流速:1.0mL/min。结果β-细辛醚、α-细辛醚的平均回收率分别为99.38%(RSD=2.25%),96.59%(RSD=2.16%)。结论方法快速准确,样品处理简便易行,可作为该药的质控指标。Objective: To determine the contents of β-Asarone and α-Asarone in Xing Nao nasal drops. Methods: HPLC was used with ODS C18 column (150mm×4.6mm, 5μm). Methanol mixture : water being 6 : 4 and Patassium biphoepate 1.4 and Dodecyl Sulphonic acid sodium salt 1.2g in 1000mL mixture served as mobile phase, detection wavelength at 257nm and flow rate being 1.0mL/min. Results: The mean recovery was 99.38 %(RSD=2.25 %) for β-Asarone and 96.59 %(RSD=2.16) for α-Asarone. Conclusion: this method is simple,rapid and accurate.

关 键 词：HPLC法 醒脑滴鼻液 Β-细辛醚 Α-细辛醚 含量测定 高效液相色谱法 中药制剂 

分 类 号：R286.0[医药卫生—中药学]