^(60)Coγ射线对南昌霉素产生菌的诱变选育  

Mutation selection by ^(60)Coγ treatment of nanchangmycin producing Streptomyces nanchangensis

作　　者：魏赛金[1] 付学琴[1] 程新[1] 涂国全[1] 

机构地区：[1]江西农业大学生物工程系,南昌330045

出　　处：《中国抗生素杂志》2002年第10期580-581,586,共3页Chinese Journal of Antibiotics

基　　金：山东胜利股份有限公司资助项目 (编号 2 0 0 0 1 1 2 0 )

摘　　要：采用不同剂量的60 Coγ射线 ,对南昌霉素产生菌南昌链霉菌 80 5 .3 116菌株的孢子进行处理。结果表明 ,7 74c/kg的诱变剂量在对孢子致死率 90 %左右时 ,具有较好的诱变效应 ;初筛摇瓶产量正变率达到 2 1 0 8% ;复筛摇瓶发酵效价比出发菌株提高 5 0 %以上的有 10株 ,占初筛菌株的 5 % ;连续四批摇瓶发酵试验平均产量比出发菌株的产量提高 5 0 %以上 ;有 6个菌株摇瓶产量分别比出发菌株提高 6 0 % ,其中 3株平均摇瓶产量比出发菌提高 70 %以上。Different dosages of 60 Coγ irradiation were used to treat the spore suspension of nanchangmycin-producing Streptomyces nanchangensis 80-5.3-116.Result showed that when 7.74 c/kg mutagenization dosage was used, which led to a 90% spore death rate, good mutagenic effect occured. Under this circumstance, the rate of positive mutation was 21.08% in the prescreening. And the average rescreening yield of 4 batch experiments was 50% higher than that of the parent strains. Six of the mutants produced a yield of 60%～70% higher than that of the parent strains.

关 键 词：南昌霉素 南昌链霉菌 ^60Co诱变 

分 类 号：Q933[生物学—微生物学]