几种实验性大鼠肾草酸钙结石模型的比较研究  

Comparison of Several Experimental Renal Calcium Oxalate Calculus Models in Rats

作　　者：曹正国[1] 刘继红[1] 段永芳[1] 李浩勇[1] 胡少群[1] 吴维[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院泌尿外科,武汉430030

出　　处：《华中科技大学学报（医学版）》2002年第5期556-559,563,共5页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目 (No. 39970 891)

摘　　要：采用几种不同诱石剂诱导大鼠肾草酸钙结石模型成功后 ,检测各组大鼠肾功能、草酸钙结晶、 2 4 h尿总钙和草酸 ,以比较几种目前国内外常用的实验性大白鼠肾草酸钙结石模型 ,设计并寻找一种成石效果好、简便、稳定的结石模型。结果发现 :乙二醇和 1α (OH) Vit D3诱导模型组的尿钙、肾钙明显高于其它模型组 ,肾组织可见大量草酸钙结晶沉积。乙二醇和氯化铵诱导的模型组与乙二醇和 1α (OH) Vit D3诱导的模型组情况类似。研究显示 :乙二醇和1α(OH) Vit D3与乙二醇和氯化铵诱导大鼠肾草酸钙结石形成效果显著、简便。Several models of renal calcium oxalate calculus in rats were compared and a simple and stable calculus model having satisfactory effects of stone formation was designed. After several renal calcium oxalate calculus models in rats were developed with calculus inducers, the renal function, calcium oxalate crystallization, 24h urinary calcium and oxalate excretion were measured. Results showed that in the model group induced by ethylene glycol (EG) and Vitamin D 3 [1α(OH)VitD 3 ], the renal tissue calcium and urinary calcium excretion were significantly higher than other model groups and calcium oxalate crystals were distributed in renal tissue. The model group induced by EG plus ammonia chloride (AC) was similar to the model by EG plus Vitamin D 3 . The method of renal calcium oxalate calculus models induced by EG plus Vitamin D 3 or by EG plus AC was simple and stable.

关 键 词：尿石 草酸钙 大鼠 动物疾病模型 

分 类 号：R691.4[医药卫生—泌尿科学] R－332[医药卫生—外科学]