共聚焦显微镜观测鼠脑损伤后早期的微循环变化  

Early Changes of Microcirculation in Rats Brain After Injury

作　　者：欧绍武[1] 王义宝[1] 刘薇[1] 王运杰[1] 吴安华[1] 杨国瑞[1] 

机构地区：[1]中国医科大学第一临床学院神经外科,辽宁沈阳110001

出　　处：《中国医科大学学报》2002年第5期354-355,358,共3页Journal of China Medical University

摘　　要：目的 :对鼠脑皮质微循环损伤前、后的变化进行活体的、动态的观测。方法 :将戊巴比妥麻醉下的 10只大鼠制成开放性骨窗模型 ,保持硬脑膜完整。荧光素标记血浆后 ,应用共聚焦激光扫描显微镜观测血管口径、形状及血流速率在脑损伤前后的变化。结果 :伤后 10s即出现血管痉挛 ,随后管径逐渐增粗 ,30min后基本恢复伤前水平。伤后 10s血流量降至最低 (下降 33.6 % ) ,以后有所回升 ,但始终低于伤前水平 ,处于缺血状态。结论 :损伤后早期降颅压对改善微循环具有重要的临床意义。Objective: The study was designed to examine the local cerebral blood flow, diameter of microvessels, and blood flow velocity before, during, and after brain injury. Methods: Rats (n=10) were prepared for opening cranial window with dura intact. The plasma was marked by fluorescein. The diameter of blood vessel, blood flow velocity ,shape of microvessel were measured before injury. The changes were recorded 10 seconds, 5 minutes, 20 minutes, 30 minutes, and 60 minutes after injury. Results: Ten seconds after injury, the microvessel was spasmed, then enlarged slowly, and restored 30 minutes later. The injury region became ischemic. Conclusion: The microvessel spasmed, and cerebral blood flow decreased at the early stage after injury. Decreasing the intracranial pressure has a great significance to microcirculation.

关 键 词：共聚焦激光扫描显微镜 大鼠 脑损伤 微循环 

分 类 号：R651[医药卫生—外科学]