HPLC测定磷酸咯萘啶片中主药的含量  

DETERMINATION OF MALARIDINE PHOSPHATE BY HPLC

作　　者：周继春 王雪芹 王巨才 

机构地区：[1]河南省药品检验所,河南郑州450003

出　　处：《华西药学杂志》2002年第5期363-364,共2页West China Journal of Pharmaceutical Sciences

摘　　要：目的　建立测定磷酸咯萘啶含量的HPLC方法。方法　选用DiamonsilC1 8(2 50mm× 4 .6mm ,5μm)色谱柱 ,0 .0 8mol·L- 1 磷酸二氢钾 -乙腈 (85∶1 5)为流动相 (含 0 .1 %三乙胺 ,pH3 .0 ) ;检测波长 2 78nm ;流速为 1 .0ml·ml- 1 ;进样量 2 0 μl。 结果　磷酸咯萘啶在浓度 5 .0 8～ 40 6 .4μg·ml- 1 范围内线性关系良好 ;回归方程为A =- 1 2 91 8+1 8657C ,r =0 .9999;平均回收率为 99.8% ,RSD =0 .8% (n =5)。结论　本法简便、灵敏。OBJECTIVE To establish an HPLC method for the determination of malaridine phosphate. METHODS The HPLC system consisted of a Diamonsil C 18 column(250 mm×4.6 mm,5 μm) and 0.08 mol·L -1 potassium dihydrogen phosphate-acetonitrile (85∶15,containing 0.1% triethylamine,pH 3.0) as the mobile phase.The flow rate was 1.0 ml·min -1 and detection wavelength was 278 nm;RESULTS The linear range was 5.08～406.4 μg·ml -1 , r=0.9999; the average recovery was 99.8%, RSD=0.8%(n=5). CONCLUSION This method was simple, sensitive and accurate.

关 键 词：HPLC 磷酸咯萘啶 含量 

分 类 号：R927.2[医药卫生—药学]