豚鼠发育过程前庭上皮ERK1/2的表达  

ERK1/2 expression in vestibular epithelium during the development of guinea pig

作　　者：胡金玮[1] 王锦玲[1] 黄维国[1] 邱建华[1] 姜鸿彦[1] 乔莉[1] 

机构地区：[1]第四军医大学西京医院全军耳鼻咽喉专科中心,陕西西安310033

出　　处：《第四军医大学学报》2002年第19期1734-1737,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金资助项目 (3 9970 75 2 )

摘　　要：检测胞外信号调控蛋白激酶 1/ 2(extracellular signal regulated kinase,ERK1/ 2 )在豚鼠发育过程前庭上皮的表达和分布特点 ,并探讨其生物学特性及意义 .方法　利用免疫组织化学的方法 ,以 ERK1,ERK2抗体为标记物 ,检测发育期豚鼠前庭器中的表达情况及分布特点 ;利用 Western blot方法检测 ERK1/ 2的活性变化 .结果　ERK1/ 2可在豚鼠前庭上皮细胞中表达 ,随年龄增加而逐渐减少 .ERK1/ 2的活性随年龄的增长而逐渐降低 ,且 ERK2的活性高于 ERK1.结论　正常豚鼠前庭上皮中有 ERK1/ 2表达 ,其表达量及活性与细胞增殖、分化相一致 。AIM To investigate the the expression of ERK1/2 of vestibular epithelium in normal developing guinea pigs so as to elucidate the function and biological character of ERKs. METHODS Guinea pigs at different ages were studied. The expression of ERK1/2 was detected by anti ERK1 and anti ERK2 immunocytochemistry, while the activation of ERKs was detected by Western blot. RESULTS Immuno reactivi ties of ERK1 and ERK2 were detected in the vestibular sensory epithelium of guinea pigs, and the quantity of staining was found to decrease with aging. The activation of ERKs also declined with aging, and the activation of ERK2 was higher than that of ERK1. CONCLUSION ERK1/2 were expressed in vestibular sensory epithelium, and the expressions were concomitant with cell proliferation and differentiation during the development of guinea pigs.

关 键 词：前庭 感觉上皮 ERK1/2 细胞增殖 

分 类 号：R764[医药卫生—耳鼻咽喉科]