银杏叶制剂对一侧迷路切除后前庭内侧核区突触素表达的影响  

Influence of Ginaton on expression of synaptophysin in the medial vestibular nucleus after unilateral labyrinthectomy

作　　者：王锦玲[1] 吴子明[1] 王尔贵[2] 邓瑶珠[1] 刘顺利[1] 

机构地区：[1]第四军医大学西京医院全军耳鼻咽喉专科中心,陕西西安710033 [2]解放军第414医院耳鼻咽喉科

出　　处：《第四军医大学学报》2002年第19期1751-1753,共3页Journal of the Fourth Military Medical University

基　　金：全军"九五"医学科研规划科研基金资助项目 (98M0 5 1)

摘　　要：目的　观察银杏叶制剂对左侧迷路切除后前庭内侧核 (medial vestibular nucleus,MVN )区突触素(synaptophysin)表达的影响 .方法　健康成年豚鼠 15只 ,随机分 3组 :正常对照组、迷路切除 (U L)组和 UL+金纳多(EGb)组 ,ip EGb,5 0 mg· kg- 1 · d- 1 ,共 30 d.左侧迷路切除后 ,不同存活时间行前庭内侧核突触素二步法免疫组化染色 .结果　一侧迷路切除 (unilateral labyrinthectomy,UL )后 10h,受损侧 MVN突触素免疫反应强度降低 ,2 0 d有所增加 (P<0 .0 5 ) ,90 d明显恢复至正常 (P>0 .0 5 ) .未损侧突触素免疫反应强度在不同时间段与正常对照无显著差别 .U L+金纳多 (EGb)组受损侧 ,术后 10 h及 2 0 d较同期 UL组 MVN突触素免疫反应强度高 (16 7.0± 2 .7,172 .5± 2 .0 vs 15 9.9±1.9,16 9.2± 3.1,P<0 .0 5 ) ,但术后 90 d与正常对照无显著差别 (P>0 .0 5 ) .结论　U L+EGb组突触密度恢复较 UL 组快 .前庭代偿及银杏叶制剂促进前庭代偿与前庭核区结构的调整有关 .AIM To observe the influence of Ginaton on the expression of synaptophysin in the medial vestibular nucleus (MVN) following unilateral labyrinthectomy (UL). METHODS 15 guinea pigs were in 3 random groups: the control group, the UL group, UL + Ginaton (EGb) group, ip Ginaton, 50 mg·kg -1 ·d -1 , for 30 d. Following left labyrinthectomy, the immunohistological staining of synaptophysin was performed. RESULTS 10 h after UL, the immunoreactive intensity of synaptophysin declined in the lesioned side. The intensity increased slightly 20 days later ( P < 0.05) and recovered significantly 90 days after UL, and no significant difference in the intensity was found in the control ( P >0.05). In the UL+EGb group, the intensity of synaptophysin was stronger than that in the UL at 10 h and 20 d (167.0±2.7, 172.5±2.0 vs 159.9±1.9, 169.2±3.1, P <0.05) . But at 90 d, the UL+EGb was intense than UL and had no significant difference compared with the control ( P >0.05). CONCLUSION In the UL+EGb group the synaptic density recovered quickly. Vestibular compensation and the enhancing effect of Ginaton is involved in the structural resconstruction in the MVN.

关 键 词：迷路切除 前庭内侧核 前庭代偿 豚鼠 突触素 

分 类 号：R764[医药卫生—耳鼻咽喉科]