西藏牦牛和黑白花奶牛生长激素基因AluⅠ多态性的比较研究  

PCR-RFLP at growth hormone gene in yak and Holstein

作　　者：陈桂芳[1] 李齐发[1] 强巴央宗[2] 土登 谢庄[1] 

机构地区：[1]南京农业大学动物科技学院 [2]西藏农牧学院牧医系,西藏林芝860000 [3]西藏林芝种牛场

出　　处：《畜牧与兽医》2002年第11期15-16,共2页Animal Husbandry & Veterinary Medicine

摘　　要：利用聚合酶链式反应 (PCR)技术 ,测定了西藏牦牛和黑白花奶牛生长激素基因的AluⅠ RFLP (限制性内切酶片段长度多态性 ) ,并比较了2牛群的基因频率。在西藏牦牛和黑白花奶牛中 ,等位基因A的频率分别为 0 935和 0 90 5 ,等位基因B的频率分别为 0 0 65和 0 0 65 ,卡方检验差异不显著 (P >0 0 5)。By polymerase chain reaction restriction fragment length polymorphism (PCR RFLP), growth hormone gene in Holstein and yak of Tibet was tested.A polymorphic AluⅠsite in the fifth exon at position 2141 was verified, one could be designated allele A and the other allele B. In yak and Holstein, the allele frequencies of A were 0 905 and 0 935 respectively, and those of B were 0 095 and 0 065 respectively without significant difference(P>0 05).

关 键 词：西藏牦牛 黑白花奶牛 生长激素基因 ALU I多态性 

分 类 号：S823[农业科学—畜牧学]