基于细胞生物学方法比较5种不同组织来源间充质干细胞的特征  

Comparing biological characteristics of five kinds of mesenchymal stem cells

作　　者：刘超 张丽君 陶昊[3] 杜心洁 王芮 李文菁 丁淑梅 王怡 姜磊 黄玉香 Liu Chao;Zhang Lijun;Tao Hao;Du Xinjie;Wang Rui;Li Wenjing;Ding Shumei;Wang Yi;Jiang Lei;Huang Yuxiang(Linyi Women and Children Hospital,Linyi 276000,Shandong Province,China;Allcare Biomedical Development,Qingdao 266000,Shandong Province,China;The Affiliated Hospital of Qingdao University,Qingdao 266000,Shandong Province,China)

机构地区：[1]临沂市妇女儿童医院,山东省临沂市276000 [2]青岛奥克生物开发有限公司,山东省青岛市266000 [3]青岛大学附属医院,山东省青岛市266000

出　　处：《中国组织工程研究》2019年第33期5334-5340,共7页Chinese Journal of Tissue Engineering Research

基　　金：山东省重点研发计划项目(2018GSF118121),项目负责人:张丽君~~

摘　　要：背景:间充质干细胞的来源以及分离方法越来越多样,充分认识到这些间充质干细胞的异同,将有助于间充质干细胞在临床中更加合理规范的应用。目的:比较不同组织来源间充质干细胞的生物学特征。方法:采集剖宫产产妇在生育后脱落的脐带、胎盘以及部分脂肪组织,采用胰酶消化法或组织块贴壁法培养出底蜕膜间充质干细胞、绒毛膜间充质干细胞、脂肪间充质干细胞、脐带胎盘连接处间充质干细胞、脐带间充质干细胞,所用的干细胞培养基有3 种:含胎牛血清的干细胞培养基、含脐血裂解物的干细胞培养基、含无血清添加物的干细胞培养基。检测不同培养条件下的细胞直径、表面标志物以及多向分化能力,检索GEO数据库分析不同间充质干细胞的基因表达情况。结果与结论:①不同培养条件影响细胞大小和细胞周期,其中无血清培养体系培养的间充质干细胞直径偏小,而脐血裂解物干细胞培养基能够增加胎盘源间充质干细胞的直径;②5 种间充质干细胞有类似的表面标志物,都具有成骨、成软骨和成脂肪分化能力,其中脂肪间充质干细胞成脂肪分化能力更强;③脂肪间充质干细胞相比于与其他4 种干细胞在基因表达上存在较大差异,具有更复杂细胞基质成分。BACKGROUND: Various methods have been introduced to isolate mesenchymal stem cells of different origins. Afull understanding of similarities and differences among mesenchymal stem cells of different origins will contributeto the rational and standardized application of mesenchymal stem cells in clinical practice.OBJECTIVE: To compare the biological characteristics of mesenchymal stem cells of different origins.METHODS: We collected umbilical cord, placenta and part of adipose tissues that were shed from parturients after cesarean delivery, anddecidua basalis-derived mesenchymal stem cells, chorionic villi-derived mesenchymal stem cells, adipose-derived mesenchymal stem cells,mesenchymal stem cells at the junction between umbilical cord and placenta, and umbilical cord mesenchymal stem cells were cultured bytrypsinization or tissue explant method. There were three kinds of stem cell culture media, including stem cell culture medium containing fetalbovine serum, stem cell culture medium containing cord blood lysate, and serum-free stem cell culture medium. Cell diameter, surfacemarkers and multi-directional differentiation ability under different culture conditions were examined. Gene expression of differentmesenchymal stem cells was analyzed by retrieval of the GEO database.RESULT AND CONCLUSION: Under different culture conditions, there were no differences in cell size and cell cycle. The diameter ofmesenchymal stem cells cultured in serum-free culture system was decreased, while the diameter of placenta-derived mesenchymal stemcells cultured in the stem cell culture medium containing cord blood lysate increased. Mesenchymal stem cells of five origins had similarsurface markers, osteogenic, chondrogenic and adipogenic abilities, in which adipose-derived mesenchymal stem cells have strongeradipogenic ability. Moreover, adipose-derived mesenchymal stem cells are different from other kinds of stem cells in term of gene expression,and have complex extracellular matrix.

关 键 词：间充质干细胞 组织块培养法 胰酶消化法 脐带胎盘连接处间充质干细胞 脐带间充质干细胞 脂肪间充质干细胞 底蜕膜间充质干细胞 绒毛膜间充质干细胞 

分 类 号：R459.9[医药卫生—治疗学] R394.2[医药卫生—临床医学]