在牙周炎样本中长链非编码RNA LINC00511表达增高并促进破骨细胞增殖  

Increased expression of long-chain non-coding RNA LINC00511 in periodontitis promotes osteoclast proliferation

作　　者：赵祥宇[1] 张桂荣[2] 郭传波[3] 史春 吴刘中 Zhao Xiangyu;Zhang Guirong;Guo Chuanbo;Shi Chun;Wu Liuzhong(Department of Prosthodontics,Shenyang 110002,Liaoning Province,China;Department of Orthodontics,Shenyang 110002,Liaoning Province,China;Department of Oral and Maxillofacial Surgery,Shenyang 110002,Liaoning Province,China;Dalian Medical University School of Stomatology,Dalian 116041,Liaoning Province,China;Department of Periodontology,Stomatology Hospital of Shenyang,Shenyang 110002,Liaoning Province,China)

机构地区：[1]沈阳市口腔医院修复科,辽宁省沈阳市110002 [2]沈阳市口腔医院正畸科,辽宁省沈阳市110002 [3]沈阳市口腔医院口腔颌面外科,辽宁省沈阳市110002 [4]沈阳市口腔医院牙周科,辽宁省沈阳市110002 [5]大连医科大学口腔医学院,辽宁省大连市116041

出　　处：《中国组织工程研究》2019年第33期5360-5365,共6页Chinese Journal of Tissue Engineering Research

基　　金：辽宁省自然科学基金(20180550563),项目负责人:史春~~

摘　　要：背景:LINC00511是一个较为全新的Lnc RNA,研究表明,lincRNA-ANRIL与牙周病的发生及发展密切相关。目的:观察LINC00511在牙周炎中的表达情况及对破骨细胞的增殖及分化的影响。方法:研究方案经沈阳市口腔医院伦理委员会批准,参与试验的患病个体及其家属对试验方案完全知情同意。取牙周炎患者及正畸拔牙患者的正常牙周膜组织(对照组),采用Realtime PCR检测牙周炎患者LINC00511、抗酒石酸酸性磷酸酶及cathepsinK的表达;取第3代的破骨前体细胞RAW264.7细胞进行破骨细胞诱导并鉴定。将LINC00511-si RNA转染破骨细胞,以未转染的破骨细胞为对照,Realtime PCR检测转染效率。采用100μg/L的脂多糖作用于RAW264.7细胞0,24和48h,检测LINC00511的表达;采用CCK8及抗酒石酸酸性磷酸酶检测LINC00511对破骨细胞的增殖及分化的影响。结果与结论:(1)人牙周炎样本中LINC00511、抗酒石酸酸性磷酸酶及cathepsinK表达均显著高于对照组;(2)脂多糖作用于RAW264.7细胞LINC00511的表达随着时间增加而增高;(3)荧光显微镜观察及Realtime PCR检测结果均显示,与对照组相比,LINC00511-si RNA转染后细胞LINC00511表达显著降低(P<0.05);(4)LINC00511促进破骨细胞的增殖及分化;(5)结果表明,在牙周炎样本中LINC00511表达增高,LINC00511可以促进破骨细胞的增殖。BACKGROUND: LINC00511 is a relatively new long-chain non-coding RNA (Lnc RNA). Studies have shown that lincRNA-ANRIL is closelyrelated to the occurrence and development of periodontal disease.OBJECTIVE: To study the expression of LINC00511 in periodontitis, and to explore LINC00511 effects on osteoblast proliferation anddifferentiation.METHODS: The study protocol was approved by the Ethics Committee of Stomatology Hospital of Shenyang. Patients and their relativeswere fully informed of study protocol and informed consent was signed prior to the inception of the trial. Periodontal ligament tissues wereextracted from patients with periodontitis and patients undergoing orthodontic extraction (control group). The expression of LINC00511,tartrate-resistant acid phosphatase and cathepsin K in periodontitis patients was detected by real-time PCR. The third generation of osteoclastprecursor cells RAW264.7 were induced to differentiate into osteoclasts and identified. LINC00511-siRNA was transfected into osteoclasts,and non-transfected osteoclasts were used as control. Transfection efficiency was detected by real-time PCR. RAW264.7 cells were culturedwith 100 μg/L lipopolysaccharide for 0, 24, and 48 hours, and the expression of LINC00511 was detected. After LINC00511 transfection,proliferation and differentiation of osteoclasts were detected by cell counting kit-8 and tartrate-resistant acid phosphatase, respectively.RESULTS AND CONCLUSION: The levels of LINC00511, tartrate-resistant acid phosphatase and cathepsin K in human periodontitissamples were markedly higher than those in the control group. The expression of LINC00511 was time-dependently increased in osteoclastsafter lipopolysaccharide treatment. Findings from fluorescence microscopy and real-time PCR showed that the expression of LINC00511 wassignificantly decreased after LINC00511-siRNA transfection compared with the control group (P < 0.05). LINC00511 promoted theproliferation and differentiation of osteoclasts. To conclude, the expression of LINC00511 is in

关 键 词：破骨细胞 牙周炎 RAW264.7细胞 增殖 LINC00511 Lnc RNA 

分 类 号：R446[医药卫生—诊断学] R496[医药卫生—临床医学]