大鼠眼睑运动加速度及角加速度的三维动态定量测量  

Three-Dimensional Dynamic Quantitative Measurement of Eyelid Linear and Angular Acceleration Movement in Rats

作　　者：庄园 冯国栋 高志强 ZHUANG Yuan;FENG Guodong;GAO Zhiqiang(Department of Otorhinolaryngology,Peking Union Medical College Hospital,CAMS and PUMC,Beijing,China,100730;Department of Otorhinolaryngology,Beijing hospital,Beijing,China,100730)

机构地区：[1]中国医学科学院北京协和医院耳鼻咽喉科北京协和医学院,北京100730 [2]北京医院耳鼻咽喉科,北京100730

出　　处：《中华耳科学杂志》2019年第4期457-464,共8页Chinese Journal of Otology

基　　金：国家自然科学基金(No.81170906和81341031);国家十二五科技支撑计划(No.2012BAI12B01)~~

摘　　要：目的探索面瘫大鼠眼睑运动三维动态测量,尤其是加速度及角加速度指标,为定量评价面瘫大鼠模型的眼睑运动提供方法。方法建立面瘫大鼠模型54只,使用自主设计的三维动态面肌运动测量系统采集大鼠面部与眼睑运动相关标记点的运动,计算其运动加速度及角加速度指标,统计分析其规律。结果实验纳入的加速度及角加速度,实验组的面瘫侧与健侧,或实验组与对照组间有明显统计学差异;1.单侧测量指标中:(1)上睑中点相对下睑中点运动加速度最大值,对照组为左侧1949mm/s^2,右侧为1895mm/s^2,无明显统计学差异(P=0.98>0.05),右侧/左侧比值为0.99;实验组左侧为1694mm/s^2,右侧为452mm/s^2,有明显统计学差异(P=0.00<0.05),右侧/左侧比值为0.25,与对照组比值的分布有明显统计学差异(P=0.00<0.05)。小于上睑中点相对于内眦点运动加速度最大值及下睑中点相对于内眦点运动加速度最大值。(2)内眦角的角加速度最大值,对照组为左侧12416°/s^2,右侧为12813°/s^2,无明显统计学差异(P=0.81>0.05),右侧/左侧比值为1.00;实验组左侧为12532°/s^2,右侧为894°mm/s^2,有明显统计学差异(P=0.00<0.05),右侧/左侧比值为0.07,与对照组比值有明显统计学差异(P=0.00<0.05)。2.双侧测量指标中:(1)右侧上睑中点相对于左侧上睑中点运动加速度的最大值,对照组为2472mm/s^2,实验组为2461mm/s^2,无明显统计学差异(P=0.53>0.05);(2)以鼻尖为顶点,左侧上睑中点及右侧上睑中点为边的角,其角加速度最大值,对照组为5007°/s^2,实验组为3328°/s^2,有明显统计学差异(P=0.00<0.05。结论该系统可适用于测量大鼠等小型实验动物的眼睑运动的加速度和角加速度,实现小型动物的三维、定量、动态、连续测量。Objective To measure three-dimensional eyelid movements in rats,especially the dynamic changes oflinear and angular acceleration over time,in order to provide a method of quantitative evaluation of eyelid movement ina rat facial paralysis model.Methods Facial paralysis was established on right side in 54 rats.Movement of eyelid markerswere measured using a three-dimensional dynamic quantitative analysis system of facial motion(3-D ASFM).Eyelidmidpoint linear acceleration and angular acceleration at the medial canthus were calculated and compared between leftand right sides and with rats without facial paralysis.Results Unilateral measurements:(1)Peak linear acceleration ofthe upper eyelid midpoint relative to the lower eyelid midpoint was 1949mm/s^2 on left side and 1895mm/s^2 on rightside in rats without facial paralysis(right/left=0.99,P=0.98>0.05),whereas it was 1694mm/s^2 on left and 452mm/s^2 on right in rats with facial paralysis(right/left=0.25,P=0.00<0.05).It is smaller than the maximum acceleration ofthe upper eyelid midpoint relative to the inner canthus point and the lower eyelid midpoint relative to the inner canthus point.(2)Maximum angular acceleration at the medial canthus angle was 12416°/s^2 on the left side and 12813°/s^2 on the right side in rats without facial paralysis(right/left=1.0,P=0.81>0.05);and 12532°/s^2 on the left side and 894°mm/s^2 on the right side in rats with facial paralysis(right/left=0.07,P=0.00<0.05).2.Side-side relative measurements:(1)The maximum acceleration of the right upper eyelid relative to the left upper eyelid was 2472mm/s^2 in rats without paralysis and 2461mm/s^2 in those with paralysis(P=0.53>0.05).(2)With the tip of nose as the apex,and the midpoint of the left and right upper eyelids as the sides,maximum angular acceleration was 5007°/s^2 in rats without faical paralysis and 3328°/s^2 in those with facial paralysis(P=0.00<0.05).Conclusions The three-dimensional dynamic quantitative analysis system of facial motion can be used to measure linear and angula

关 键 词：面瘫 眼睑运动 三维测量 定量测量 大鼠 

分 类 号：R764[医药卫生—耳鼻咽喉科]