复方浙贝颗粒联合顺铂对L1210/CDDP移植瘤小鼠的抑瘤作用及相关凋亡蛋白的影响  

Tumor suppression of the compound zhebei granules and cisplatin in the mice with L1210/CDDP transplanted tumor and the impacts on relevant apoptotic proteins

作　　者：吕鹏 赵欢 石凤芹 陈信义 侯丽 LV Peng;ZHAO Huan;SHI Feng-qin;CHEN Xin-yi;HOU Li(Dongzhimen Hospital of Beijing University of Traditional Chinese Medicine,Beijing 100700)

机构地区：[1]北京中医药大学东直门医院

出　　处：《世界中西医结合杂志》2019年第7期894-897,901,共5页World Journal of Integrated Traditional and Western Medicine

基　　金：北京市科技计划“十病十药研发”项目(Z151100003815027)

摘　　要：目的探讨复方浙贝颗粒联合顺铂(CDDP)对L1210/CDDP移植瘤小鼠的抑瘤作用及瘤体组织相关凋亡蛋白的影响。方法将急性淋巴细胞白血病多药耐药细胞株L1210/CDDP细胞接种于DBA/2小鼠腋前皮下构建多药耐药移植瘤模型,成模后按随机数字表法将移植瘤小鼠分成模型组、阳性药物(CDDP)组、高剂量CZBG联合CDDP组、中剂量CZBG联合CDDP组、低剂量CZBG联合CDDP组、中剂量CZBG组,分组当天给药;治疗结束后,计算实验各组的抑瘤率,应用免疫组织化学检测各组移植瘤的BCL-2及BAX蛋白表达情况。结果与模型组及顺铂组比较,各剂量CZBG联合CDDP组均能提高急性淋巴细胞白血病耐药细胞株L1210/CDDP移植瘤的抑制率(P<0.05);与阳性药CDDP组比较,高剂量CZBG联合CDDP组具有较高的抑瘤率(P<0.05)。与模型组及阳性药CDDP组比较,中、高剂量CZBG联合CDDP组能降低BCL-2表达(P<0.05),提高BAX表达及BAX/BCL-2比值(P<0.05)。结论CZBG联合CDDP能提高急性淋巴细胞白血病耐药细胞株移植瘤的肿瘤抑制率,其机制可能是通过调节BAX/BCL-2凋亡途径来逆转多药耐药性,从而增加肿瘤细胞对药物的敏感性,起到协同增效的作用。Objective To explore the tumor suppression of the compound zhebei granules and cisplatin(CZBG)in the mice with L1210/CDDP transplanted tumor and the impacts on relevant apoptotic proteins of tumor tissue.Methods L1210/CDDP cells,the multidrug resistance cell strain of acute lymphoblastic leukemia,were inoculated subcutaneously in DBA/2 mice to establish a multidrug-resistance transplanted tumor model.After modeling,according to the random number table,the transplanted tumor mice were divided into a model group,a positive drug group(CDDP)group,a high-dose CZBG plus CDDP group,a middle-dose CZBG plus CDDP group,a low-dose CZBG plus CDDP group and middle-dose CZBG group.The drugs were given in individual group.After treatment,the tumor inhibition rate of each experimental group was calculated.IHC method was adopted to determine the protein expressions of BCL-2 and BAX of transplanted tumor in each group.Results Compared with the model group and the cisplatin group,In every CZBG plus CDDP group,the inhibition rate of transplanted tumor induced by L1210/CDDP was all improved(P<0.05).Compared with the positive CDDP group,in the high-dose group of CZBG plus CDDP,the tumor inhibition rate was higher(P<0.05).Compared with the model group and the positive CDDP group,BCL-2 expression was reduced,BAX expression and the ratio of BAX to BCL-2 were all increased in the middle and high dose group of CZBG plus CDDP group(P<0.05).Conclusion The combined medication of CZBG and CDDP improves the tumor inhibition rate of L1210/CDDP transplanted tumor and the effect mechanism may be related to the reversion of multidrug resistance by regulating BAX/BCL-2 apoptosis so as to increase the sensitivity of tumor cells to the drug and induce synergistic interaction.

关 键 词：复方浙贝颗粒 顺铂 L1210/CDDP细胞 细胞凋亡 急性淋巴细胞白血病 

分 类 号：R285.5[医药卫生—中药学]