苍耳亭抑制肝癌HepG2体内外药效研究  

Anticancer effect of xanthium in vitro and in vivo in HepG2 of hepatocellular carcinoma

作　　者：蔡亚云[1] 吴育[1] 杨水英[1] 缪婧[1] 曹刚[1] 陈刚[1] CAI Ya-yun;WU Yu;YANG Shui-ying;MIAO Jing;CAO Gang;CHEN Gang(Nantong Hospital of Chinese Medicine,Nantong Jiangsu 226001)

机构地区：[1]南通市中医院

出　　处：《世界中西医结合杂志》2019年第7期946-949,共4页World Journal of Integrated Traditional and Western Medicine

基　　金：江苏省南通市青年基金(WQ2016056);江苏省药学会-奥赛康医院药学科研项目(A201720);南通市市级科技项目(MS12017021-6)

摘　　要：目的通过体外肿瘤细胞抑制实验及体内抗肿瘤实验,探讨苍耳亭体内外抗人肝癌细胞HepG2的作用。方法体内通过构建裸鼠肝癌自发转移模型,并随机分成空白对照组、苍耳亭低、高剂量组,连续腹腔注射2周,完整剥离肿瘤组织,称取瘤质量,计算抑瘤率,并且剥取主要脏器,计算脾指数与胸腺指数;体外通过MTT、Transwell实验初步观察苍耳亭对癌细胞侵袭迁移的作用。结果体内实验结果显示苍耳亭低剂量组与高剂量组的抑瘤率分别为17.61%和39.57%,具有较好的抑制肿瘤活性(P<0.05,P<0.01),并且高低剂量组间比较差异有统计学意义(P<0.05)。体外苍耳亭抑制HepG2细胞增殖效果明显,与空白对照组DMSO比较,16μM时诱导肿瘤细胞凋亡差异有统计学意义(P<0.01),其IC 50值为29.04μM。同时,Transwell实验显示也能抑制体外癌细胞侵袭。结论苍耳亭体内外对肝癌HepG2细胞均具有较强的抑制作用,具有浓度依赖性。Objective To explore the anticancer effect of xanthium in vitro and in vivo in HepG2 of hepatocellular carcinoma through the in vitro experiment of carcinoma cell inhibition.Methods The models of spontaneous metastasis of hepatocelular carcinoma were established in naked mice and they were randomized into a blank control group,a low-dose xanthatin group and a high-dose xanthatin group.The intraperitoneal injection was given continuously for 2 weeks.The tumor tissue was exfoliated completed,and thus tumor mass was weighted and the tumor inhibition was calculated.The main organs were separated and the spleen index and thymus index were calculated.In vitro,with MTT and Transwell experiments,the effect of xanthatin on cellular invasion and migration was observed.Results The in vivo experiment results indicated that the tumor inhibition rates in the low-dose xanthatin group and the high-dose group were 17.61%and 39.57%respectively,presenting the satisfactory activity of tumor inhibition(P<0.05,P<0.01).The inhibition rate was different significantly between the high-dose group and the low-dose group(P<0.05).The in vitro experiment presented the remarkable effect of the inhibition of HepG2 proliferation.In DMSO comparison with the blank control group,the difference in the induced tumor cell apoptosis was significant at 16μM(P<0.01)and IC 50 value was 29.04μM.At the same time,the inhibition in vitro of cancer cell invasion was also presented in Transwell experiment.Conclusion Xanthatin presents the strong inhibition of HepG2 of hepatoceluar carcinoma in vitro and in vivo and the concentration dependence.

关 键 词：苍耳亭 裸鼠肝癌自发转移模型 凋亡 侵袭与转移 

分 类 号：R285.5[医药卫生—中药学]