酪氨酸微波水热法制备碳点及其在Fe3+检测和荧光标记方面的应用  

Microwave-assisted Hydrothermal Synthesis of Carbon Dots Based on Tyrosine and Their Application in Ion Detection and Bioimaging

作　　者：黄贺 李春光[1] 施展[1] 冯守华[1] HUANG He;LI Chunguang;SHI Zhan;FENG Shouhua(State Key Laboratory of Inorganic Synthesis and Preparative Chemistry,Jilin University,Changchun 130012,China)

机构地区：[1]吉林大学无机合成与制备化学国家重点实验室

出　　处：《高等学校化学学报》2019年第8期1579-1585,共7页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:21771077,21771084,21621001);国家重点研究发展计划项目(批准号:2016YFB0701100);高等学校学科创新引智计划(批准号:B17020);吉林大学高层次科技创新团队项目资助~~

摘　　要：以酪氨酸为原料,乙二胺为钝化剂,采用绿色、简便的微波辅助水热法制备了尺寸均一、分散性好的碳点.通过调节乙二胺的加入量可以在一定程度上对碳点尺寸进行调节.所得碳点可以通过改变激发波长实现蓝光到绿光的转变,且荧光强度随激发光波长的红移也保持了一定的强度.所得碳点对Fe^3+具有很好的选择性猝灭,被用于检测Fe^3+时检测限为0.82 nmol/L,检出限为2.98 nmol/L.所制备碳点具有良好的生物相容性和低细胞毒性,被用于标记AD293细胞时标记位置为细胞质.Cabon dots(CDs) were synthesized utilizing tyrosine as carbon source, ethanediamine as passivant through a green microwave-assisted hydrothermal way. The size of CDs can be adjusted by the dose of ethanediamine. The as-prepared CDs have uniform size and good dispersibility, showing blue or green fluorescence. The as-prepared CDs can change their fluorescence(from blue to yellow) by changing the excitation light and the emission light can be kept without large decay. The as-synthesized CDs have good selectivity on the detection of Fe ^3+ with a limit of detection(LOD) of 0.82 nmol/L, and a detection limit of 2.98 nmol/L. The as-synthesized CDs also have great biocompatibility and low biotoxicity, and can be utilized as a probe to label AD293 cells, marking the cytoplasm of the cells.

关 键 词：碳点 微波水热法 Fe ^3+ 检测 细胞标记 

分 类 号：O613.7[理学—无机化学] O648.12[理学—化学]