盐胁迫条件下不同基因型籼稻表达谱芯片数据分析  

Data Analysis of Expression Profile Microarray of Different Genotypes of Indica Rice under Salt Stress

作　　者：刘慰华[1] 吕宏斌[1] 钱敏[2] 黄格 郑爱清[1] 崔永祯[1] 赵红[1] 朱振华[1] 丁明亮[1] Liu Weihua;Lv Hongbin;Qian Min;Huang Ge;Zheng Aiqing;Cui Yongzhen;Zhao Hong;Zhu Zhenhua;Ding Mingliang(Institute of Food Crops,Yunnan Academy of Agricultural Sciences,Kunming650205;Agricultural Economic and Informational Institute,Yunnan Academy of Agricultural Sciences,Kunming650205)

机构地区：[1]云南省农业科学院粮食作物研究所,昆明650205 [2]云南省农业科学院农业经济与信息研究所,昆明650205

出　　处：《分子植物育种》2019年第14期4513-4517,共5页Molecular Plant Breeding

基　　金：云南省应用基础研究青年项目(2017FD015);云南省技术创新人才培养计划(2015HB107)共同资助

摘　　要：盐胁迫是制约水稻产量的主要逆境因素之一,特别是对幼穗分化期的水稻产量影响尤其显著。本研究以水稻Affymetrix表达谱芯片的数据为例,应用生物信息学方法进行数据挖掘,研究幼穗分化期籼稻耐盐品种FL478和感盐品种IR29在盐胁迫处理条件下的基因差异表达情况。芯片数据分析共筛选到差异倍数在2倍及以上的基因257个,以IR29为参照,FL478中上调基因138个,下调基因119个,主要涉及到β-丙氨酸生物合成、木糖降解、脂肪酸β氧化等生物学过程。通过这些数据挖掘,初步探讨籼稻抗盐胁迫的表达调控机理。本研究为进一步揭示籼稻耐盐性分子机制提供了科学依据。Salinity is the major stress limiting rice productivity,especially during the panicle primordium differentiation stage.Taking the data of rice Affymetrix expression profile microarray as an example,data mining was carried out using bioinformatics to study the gene differential expression of salt-tolerant indica rice FL478 and salt-sensitive rice IR29 at the stage of young panicle differentiation under salt stress.A total of 257 genes with at least 2-fold differences were screened by chip data analysis.When FL478 was compared with IR29,the expression of 138 genes were up-regulated,and 119 genes were down-regulated.These genes were mainly related to biological processes just asβ-alanine biosynthesis,xylose degradation and fatty acidβ-oxidation.Through such data mining,the mechanism of expression and regulation of salt tolerance in indica rice was preliminarily discussed.This study could provide a scientific basis for further revealing the molecular mechanism of salt tolerance in indica rice.

关 键 词：盐胁迫 基因芯片 数据挖掘 

分 类 号：S51[农业科学—作物学]