辣椒属蔗糖合酶基因家族的鉴定及表达  

Identification and Expression of the Sucrose Synthase Gene Family of Genus Capsicum

作　　者：魏华伟 柴松琳 胡克玲[1] 高优洋 高朋 梁赛 陈友根[1] Wei Huawei;Chai Songlin;Hu Keling;Gao Youyang;Gao Peng;Liang Sai;Chen Yougen(College of Horticulture,Anhui Agricultural University,Hefei230036;College of Horticulture and Gardening,Yangtze University,Jingzhou434000)

机构地区：[1]安徽农业大学园艺学院,合肥230036 [2]长江大学园艺园林学院,荆州434000

出　　处：《分子植物育种》2019年第14期4537-4544,共8页Molecular Plant Breeding

基　　金：安徽省重点研究与开发计划项目(1704e0302200)资助

摘　　要：蔗糖合酶(sucrose synthase,SUS)是植物蔗糖代谢关键酶之一,在植物生长发育和抵御逆境胁迫中具有重要的作用。辣椒基因组的公布为挖掘和鉴定SUS提供了机遇。本研究基于已经公布的5个辣椒基因组数据信息,利用信息生物学方法对辣椒蔗糖合酶基因家族成员进行鉴定,分析其染色体定位、系统进化关系及表达模式。结果表明:5个辣椒基因组共编码25个SUS蛋白,每个辣椒种均编码5个成员;编码氨基酸长度在787~972 aa之间,等电点范围位于5.85~8.26之间,分子量大小为87.96~110.27kD;染色体定位发现一年生栽培辣椒5个SUS基因(Capanasus1~Capanasus5)分布于4条染色体上,其中Capanasus3和Capanasus5基因位于三号染色体;系统进化树将SUS基因分为3个不同的区组(SUSⅠ~SUSⅢ),这与模式植物拟南芥分类相一致;通过RNA-seq分析发现,2个SUS基因(Capanasus1和Capanasus2)在花、果皮和种子发育中高度表达,而在叶片中表达量很低,剩下3个基因(Capanasus3,Capanasus4和Capanasus5)在所有组织(花,叶,果皮和种子)发育中表达量较低,甚至不表达。进一步研究发现3个SUS基因(Capanasus1,Capanasus2和Capanasus3)受到多种激素(ABA,GA,IAA和JA)的诱导,而剩下的Capanasus4和Capanasus5基因几乎没有变化;此外,在逆境处理条件下,辣椒SUS基因(Capanasus1,Capanasus2和Capanasus3)呈现显著的差异表达,而Capanasus4和Capanasus5基因的表达量没有显著变化。这些结果为进一步深入研究辣椒SUS基因的功能提供了理论依据。Sucrose synthase(SUS)is one of the key enzymes in plant sucrose metabolism,and plays an important role in plant growth,development and resistance to stress.The publication of pepper genome provides an opportunity to mining and identifying SUS.Based on the published data of five pepper genomes,this study used information biology methods to identify pepper sucrose synthase gene family members,and analyzed their chromosomal location,phylogenetic relationship and expression patterns.The results showed that a total of 25 SUS proteins were encoded by 5 pepper genomes,and each pepper species encoded 5 members.The length of the encode amino acids was between 787~972 aa,the isoelectric point ranged from 5.85 to 8.26,and the molecular weight was 87.96~110.27 kD.Chromosomal localization found that five SUS genes(Capanasus1~Capanasus5)were distributed on four chromosomes.Among these genes,Capanasus3 and Capanasus5 were located on chromosome 3.The phylogenetic tree divided SUS genes into three different groups(SUSⅠ~SUSⅢ),which was consistent with the classification of model plant Arabidopsis.The RNA-seq analysis showed that two SUS genes(Capanasus1 and Capanasus2)were highly expressed in flower,peel and seed development,while the expression level in leaves was low,and the remaining three genes(Capanasus3,Capanasus4 and Capanasus5)in all these tissue that tested(flowers,peels,seeds,and placenta)had low or no expression during development.Further studies revealed that three SUS genes(Capanasus1,Capanasus2 and Capanasus3)were induced by various hormones(ABA,GA,IAA and JA),while the remaining Capanasus4 and Capanasus5 genes were almost no change.In addition,three SUS genes(Capanasus1,Capanasus2 and Capanasus3)were significantly differentially expressed under stress treatment,while there was no significant change in Capanasus4 and Capanasus5.These results provide a theoretical basis for further research on the function of SUS gene in pepper.

关 键 词：辣椒(Capsicum annumm L.) 蔗糖合酶 SUS基因家族 生物信息学 表达分析 

分 类 号：S64[农业科学—蔬菜学]