香蕉C2H2类锌指蛋白基因MaZAT10的克隆及表达分析  

Clone and Expression of the C2H2-type Zinc Finger Transcription Factor Gene MaZAT10 in Banana

作　　者：王静毅[1] 刘菊华[1] 贾彩红[1] 胡伟 金志强[1] 徐碧玉[1] Wang Jingyi;Liu Juhua;Jia Caihong;Hu Wei;Jin Zhiqiang;Xu Biyu(Key Laboratory of Biology and Genetic Resources of Tropical Crops,Ministry of Agriculture,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou,571101)

机构地区：[1]中国热带农业科学院热带作物生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室

出　　处：《分子植物育种》2019年第14期4551-4559,共9页Molecular Plant Breeding

基　　金：国家自然科学基金(31501043;31872161);海南省重点研发计划项目(ZDYF2018097);国家现代农业产业技术体系(CARS-31)共同资助

摘　　要：本研究利用RT-PCR技术从巴西蕉中克隆到1个C2H2型锌指蛋白转录因子,命名为MaZAT10。香蕉MaZAT10基因开放阅读框全长732 bp,编码243个氨基酸,预测蛋白分子量为25.257 3 kD,理论等电点为7.7。香蕉MaZAT10蛋白序列包含2个C2H2型锌指结构域,在其N-端含有与核定位有关的NLS即B-box和富含亮氨酸的L-box,C-端含有一个转录抑制结构域EAR/DLN-box。系统进化分析显示,MaZAT10与马来西亚野蕉(XP_009388925.1)、油棕(XP_008792675.1)、海枣(XP_010907391.1)及菠萝(OAY84607.1)亲缘关系较近。低温、干旱和高盐胁迫处理后MaZAT10的表达水平显著上调,但在ABA处理下,MaZAT10的表达水平显著下调。本研究表明,MaZAT10可能在香蕉响应低温、干旱、高盐等非生物胁迫中起重要作用。本研究结果将为今后研究香蕉C2H2型锌指蛋白转录因子家族基因功能提供参考,并为香蕉抗逆研究及分子育种提供科学依据。In this study,a C2H2 zinc finger protein transcription factor named MaZAT10 was cloned from Canna brasiliensis by RT-PCR technology.The full-length ORF of banana MaZAT10 gene was 732 bp,coded for 243 amino acids,the predicted protein molecular weight was 25.257 3 kD and the isoelectric point is 7.7.Banana MaZAT10 protein sequence contained two C2H2-type zinc finger domains,which contained NLS related to nuclear localization at its N-terminal,that is B-box and L-box rich in leucine,and whose C-terminal contained a transcriptional inhibition domain EAR/DLN-box.Phylogenetic analysis showed that MaZAT10 was closely related to Musa acuminate subsp.Malaccensis(XP_009388925.1),Elaeis guineensis(XP_008792675.1),Phoenix dactylifera(XP_010907391.1),and Ananas comosus(OAY84607.1).The MaZAT10 expression was strongly up-regulated after the treatments of low temperature,drought,and high salt stress.However,it was significantly reduced under ABA treatment.This study suggests that MaZAT10 may play an important role in banana response to abiotic stresses such as low temperature,drought,high salt and so on.It will provide a reference for the future study of ZPT2 family gene function of banana,and provide scientific basis for banana stress resistance research and molecular breeding.

关 键 词：香蕉(Musa nana Lour.) C2H2型锌指蛋白 MaZAT10 逆境胁迫 表达分析 

分 类 号：S66[农业科学—果树学]