黄颡鱼溶血性腹水病初探  

Preliminary study on hemolytic ascites disease of Pelteobagrus fulvidraco

作　　者：杨移斌 艾晓辉[2,3] 宋怿[3] 董靖 胥宁 姜兰[1] YANG Yibin;AI Xiaohui;SONG Yi;DONG Jing;XU Ning;JIANG Lan(Pearl River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510380,China;Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Wuhan 430223,China;Key Laboratory of Control of Quality and Safety for Aquatic Products,Minisrty of Agriculture and Rural Affairs,Beijing 100141,China)

机构地区：[1]中国水产科学研究院珠江水产研究所,广东广州510380 [2]中国水产科学研究院长江水产研究所,湖北武汉430223 [3]农业农村部水产品质量安全控制重点实验室,北京100141

出　　处：《浙江农业学报》2019年第8期1239-1248,共10页Acta Agriculturae Zhejiangensis

基　　金：(原)农业部渔用药物创制重点实验室开放课题(201707);现代农业产业技术体系专项(CARS-49)

摘　　要：从濒死黄颡鱼腹水中分离到一株细菌HS01,对分离株理化特性及DNA序列进行检测,根据理化特性、16S rRNA和gyrB基因序列比对,分离株HS01为嗜水气单胞菌。人工感染该菌后发病鱼出现与自然发病类似症状,并表现强毒力,对黄颡鱼的半致死剂量为2.51×10^5 CFU·g^-1,且从病灶中分离到与感染菌一致菌株,因此确定嗜水气单胞菌是此次黄颡鱼溶血性腹水病的病原。分离株对头孢唑肟、头孢噻肟、奈替米星及氟苯尼考等10种抗生素高度敏感;对头孢拉定、链霉素、新霉素及卡那霉素等6种抗生素中度敏感;对阿莫西林、青霉素、妥布霉素及红霉素这4种抗生素耐药。本研究为黄颡鱼溶血性腹水病防控提供了理论依据。Bacterial strain HS01 was isolated from diseased Pelteobagrus fulvidraco suffered from serious hemolytic ascites,and it was identified as Aeromonas hydrophila based on biochemical reaction characters and 16S rRNA and gyrB gene sequence analyses.Artificial affected fish by intraperitoneal HS01-injection showed the same clinic signs as the diseased fish in ponds.Therefore,the isolated A.hydrophila was proved as the main pathogen,and its LD 50 to tilapia was determined as 2.51×10^ 5 CFU·g^-1 using the probabilistic graphical method.The antibiotic sensitivity test showed that the isolated was very sensitive to cefotaximee,cefotaxime,netilmicin,florfenicol,etc.,less sensitive to cefadroxil,streptomycin1,neomycin,kanamycin,etc.,and not sensitive to amoxicillin,penicillin,tobramycin,erythrocin.This study provided a theoretical basis for the prevention and control of the hemorrhagic disease caused by A.hydrophila in Pelteobagrus fulvidraco.

关 键 词：黄颡鱼 嗜水气单胞菌 分离鉴定 病理 高通量测序 

分 类 号：S941.42[农业科学—水产养殖]