白果内酯和银杏内酯B对过氧化氢诱导人角质形成细胞氧化损伤的保护作用  

Protective effects of bilobalide and ginkgolides B on oxidative damage in human keratinocytes induced by H2O2

作　　者：熊丽丹[1,2] 唐洁 李利 XIONG Li-dan;TANG Jie;LI Li(Cosmetics Safety and Efficacy Evaluation Center,West China Hospital,Sichuan University,Chengdu,Sichuan 610041,China;Sichuan Engineering Technology Research Center of Cosmetic,Chengdu,Sichuan 610041,China;Department of Dermatology,West China Hospital,Sichuan University,Chengdu,Sichuan 610041,China)

机构地区：[1]四川大学华西医院化妆品评价中心,四川成都610041 [2]四川省化妆品工程技术研究中心,四川成都610041 [3]四川大学华西医院皮肤科,四川成都610041

出　　处：《日用化学工业》2019年第8期531-536,共6页China Surfactant Detergent & Cosmetics

基　　金：四川大学华西医院学科卓越发展1·3·5工程项目;四川省科技计划资助项目(2018SZ0104);四川省科技计划资助项目(2017SZ0070)

摘　　要：通过CCK-8法测定细胞存活率,WST染色方法测定超氧化物歧化酶(SOD)活性,DTNB测定谷胱甘肽(GSH)活性,脂质氧化试剂盒检测丙二醛(MDA)含量,DCFH-DA法测定活性氧(ROS)含量,ELISA法测定炎症因子TNF-α、IL-1β和IL-6含量水平,研究了白果内酯和银杏内酯B对过氧化氢(H2O2)诱导人角质形成细胞(HaCaT细胞)的抗氧化损伤和抑制炎症因子的影响,同时利用药物数据库预测目标靶点,研究白果内酯和银杏内酯B发挥功效的机制。结果表明,白果内酯与银杏内酯B能减少H2O2诱导的SOD、谷胱甘肽过氧化物酶(GSH-Px)活性降低,抑制MDA释放增加,减少ROS的产量,减少炎症因子TNF-α、IL-1β、IL-6的生成;综合网络分析结果显示白果内酯和银杏内酯B抗炎活性呈现多分子、多靶点和多通路特点。Different concentrations of bilobalide and ginkgolides B were added to H2O2-stimulated HaCaT model.Cell viability was determined by CCK-8 method,superoxide dismutase(SOD)activity was determined by WST staining method,glutathione(GSH)activity was measured by DTNB,malondialdehyde(MDA)content was detected by lipid oxidation kit.The content of ROS was investigated via DCFH-DA method.And ELISA assays were adopted to investigate the production of TNF-α,IL-1β,and IL-6.To better understand the mechanism of the efficacy of bilobalide and ginkgolides B,TCM database(IUPHAR/BPS Guide to Pharmacology and String)was used to predict common targets of drugs.The results show that bilobalide and ginkgolides B can increase the SOD and GSH-Px activity,decrease MDA and ROS content induced by H2O2,and reduce the production of the pro-inflammatory cytokines TNF-α,IL-1βand IL-6 in H2O2-induced HaCaT cells.The drug discovery database shows that the anti-inflammatory effect of bilobalide and ginkgolides B has the characteristics as multi-molecular,multi-target and multi-pathway.

关 键 词：白果内酯 银杏内酯B 炎症因子 活性氧 体外实验 

分 类 号：TQ658[化学工程—精细化工]