一株发光细菌的分离鉴定及其对有毒物质的毒性响应  

Isolation and Identification of A Luminous Bacterium and Its Toxicity Response to Hazardous Material

作　　者：段效辉 张群 刘鹏 王颖 赵晗[3] DUAN Xiaohui;ZHANG Qun;LIU Peng;WANG Ying;ZHAO Han(Technology Center of Yantai Entry-Exit Inspection and Quarantine Bureau,Yantai 264000,China;Technology Center of Ji′nan Entry-Exit Inspection and Quarantine Bureau,Ji′nan 250000,China;Technology Center of Weifang Entry-Exit Inspection and Quarantine Bureau,Weifang 261000,China)

机构地区：[1]烟台出入境检验检疫局检验检疫技术中心,山东烟台264000 [2]济南出入境检验检疫局检验检疫技术中心,山东济南250000 [3]潍坊出入境检验检疫局检验检疫技术中心,山东潍坊261000

出　　处：《化学与生物工程》2019年第8期16-20,26,共6页Chemistry & Bioengineering

基　　金：国家重点研发计划(2017YFF0211103);原国家质检总局科技计划(2015IK200);烟台市重点研发计划(2018SFGY072)

摘　　要：从新鲜海鱼肠道内分离获得1株发光细菌Fc-11-1,通过菌落形态特征、生理生化特征、16S rDNA序列分析及系统发育树分析对其进行鉴定,初步确定发光细菌Fc-11-1为Vibrio toranzoniae。从发光细菌Fc-11-1的生长和发光强度两方面,初步探索了发光细菌Fc-11-1对食品中5种常见有毒物质百菌清、呋喃唑酮、红霉素、氯霉素、阿维菌素的毒性响应。结果表明,发光细菌Fc-11-1对5种有毒物质均有不同程度的毒性响应,可用于食品中有毒物质残留的检测。We isolated a luminous bacterium Fc-11-1 from the intestinal tract of fresh marine fish.Moreover,we identified that the luminous bacterium Fc-11-1 belongs to Vibrio toranzoniae by colony morphology,physiological and biochemical characteristics,16S rDNA sequencing analysis,and phylogenetic tree analysis.Furthermore,from the growth and luminescence intensity of the luminous bacterium Fc-11-1,we investigated the toxicity response of the luminous bacterium Fc-11-1 to five common hazardous materials,such as Chlorothaloni,Furazolidone,Chloramphenicol,Erythromycin,and Avermectin in food.The results show that the luminous bacterium Fc-11-1 has different degrees of toxicity response to five hazardous materials,which can be used for the detection of hazardous materials residues in food.

关 键 词：发光细菌 鉴定 有毒物质 毒性响应 

分 类 号：Q939.1[生物学—微生物学]