萝卜叶缘裂刻性状分子标记的开发与应用  

Development and Application of Molecular Markers for Radish Leaf Edge Cracking Traits

作　　者：张小康 张雪丽 熊秋芳 ZHANG Xiaokang;ZHANG Xueli;XIONG Qiufang(Institute of Vegetables,Wuhan Academy of Agricultural Sciences,Wuhan 430345,China;Wuhan Shubo Agricultural Technology Co.,Ltd,Wuhan 430060,China)

机构地区：[1]武汉市农业科学院蔬菜研究所,湖北武汉430345 [2]武汉蔬博农业科技有限公司,湖北武汉430060

出　　处：《广东农业科学》2019年第7期17-22,共6页Guangdong Agricultural Sciences

摘　　要：【目的】明确萝卜裂叶性状的遗传特点,开发与裂叶性状连锁的分子标记。【方法】以萝卜“武青一号”作为母本,“新洲花叶”作为父本杂交得到F1,将F1分别套袋自交和回交,产生F2和BC1,探讨裂叶性状的遗传规律。【结果】F1植株叶型表现为裂叶,BC1(F1ד武青一号”)和F2中叶全裂与叶浅裂的植株分离比分别符合1∶1和3∶1,说明萝卜叶缘裂刻的表型符合显性基因遗传模式,萝卜叶缘缺裂受单个遗传位点控制。利用BSA与AFLP技术相结合筛选与萝卜叶缘裂刻基因紧密连锁的分子标记,制备的SCAR标记引物HY-18与萝卜叶缘裂刻基因紧密连锁,其遗传距离为3.32 cM。【结论】上述标记的获得为萝卜裂叶性状的分子标记辅助育种以及裂叶基因的克隆奠定了理论基础。【Objective】The study identifies the genetic characteristics of radish leaf cracking traits and develop a molecular marker which linked to lobed leaf traits.【Method】The radish variety“WUQING No.1”(as female parent)and“XINZOUHUAYE”(as the male parent)were used to make the hybrid F1,then self-bred and backcross of F1 were made to get F2 and BC1.And the genetic characteristics of lobed leaf traits were explored.【Result】The results showed that the leaf edge of F1 was completely lobed.The separation ratio of leaf edge lobed to non-lobed plants in BC1 and F2 were 1∶1 and 3∶1,indicating that the phenotypic characteristic of radish leaf edge lobed matched with dominant genetic heredity pattern and was controlled by a single genetic locus.The BSA and AFLP were used to screen the molecular markers which were closely linked to the lobed leaf edge gene of radish,and the SCAR marker primer HY-18 was closely linked with the lobed leaf edge gene of radish,with the genetic distance of 3.32 cM.【Conclusion】The obtained markers lay a theoretical foundation for molecular marker assisted breeding and the cloning of lobed leaf genes.

关 键 词：萝卜 裂叶 分子标记 AFLP 开发与应用 

分 类 号：S631.1[农业科学—蔬菜学]