MCP-1基因-2518A/G位点多态性与2型糖尿病视网膜病变的关系研究  

Association of MCP-1 gene 2518A/G polymorphism with diabetic retinopathy in type 2 diabetes mellitus patients

作　　者：程怡 薛诚 吴金友 林海洋 赵佳佳 毛小洁 CHENG Yi;XUE Cheng;WU Jinyou(Department of Endocrinology,Wenling First People’s Hospital,Taizhou 317500,China)

机构地区：[1]温岭市第一人民医院内分泌科

出　　处：《浙江医学》2019年第15期1606-1609,共4页Zhejiang Medical Journal

基　　金：温岭市科技项目(2015C311034)

摘　　要：目的探讨单核细胞趋化蛋白-1(MCP-1)基因-2518A/G位点多态性与2型糖尿病视网膜病变的关系。方法采用聚合酶链反应-限制性酶切片段长度多态性分析技术检测192例2型糖尿病患者[其中糖尿病视网膜病变组93例(包括增殖性视网膜病变亚组45例和非增殖性视网膜病变亚组48例),糖尿病非视网膜病变组99例]和109例健康体检者(对照组)的MCP-1基因-2518A/G位点多态性。结果糖尿病视网膜病变组患者AA基因型频率高于糖尿病非视网膜病变组,差异有统计学意义(P<0.05),A等位基因频率亦高于糖尿病非视网膜病变组,差异有统计学意义(P<0.05)。增殖性视网膜病变亚组患者与非增殖性视网膜病变亚组患者的基因型频率和等位基因频率比较差异均无统计学意义(P>0.05)。logistic回归分析显示,MCP-1基因-2518A/G位点多态性及糖尿病病程与糖尿病视网膜病变的发生有关,AA基因型及糖尿病病程长是糖尿病视网膜病变发生的危险因素。结论MCP-1基因-2518A/G位点多态性与2型糖尿病患者视网膜病变发生有关,其中AA基因型可能是2型糖尿病患者发生视网膜病变的危险因素,A等位基因可能增加该人群视网膜病变的发生风险。Objective To Investigate the polymorphism of monocyte chemoattractant protein-1(MCP-1)gene 2518A/G with the susceptibility to diabetic retinopathy(DR)in type 2 diabetes mellitus(T2DM)patients.Methods One hundred and ninety two T2DMpatients were enrolled in the study,including 99 cases without DR(Non-DR group),45 cases with proliferative DR(PDR group)and 48 cases with non-proliferative DR(NPDR group);109 healthy individuals served as control group.The genotypes of MCP-1 2518A/G were determined by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).The blood glucose,glycosylated hemoglobin A1c(HbA1C),C-peptide,lipid profiles were also measured.Results The AA genotype frequencies and A allele frequencies in DR group(PDR+NPDR)were significantly higher than those in Non-DR group(P<0.05).There were no significant differences in genotype frequencies or allele frequencies between PDR and NPDR group.Logistic regression analysis showed that AA genotype and DM course were the independent risk factors for a development of diabetic retinopathy.Conclusion The AA genotype and A allele of MCP-1 2518A/G is associated with diabetic retinopathy in type 2 diabetic patients.

关 键 词：单核细胞趋化蛋白-1 基因多态性 糖尿病视网膜病变 

分 类 号：R58[医药卫生—内分泌]